The Locust Standard Brain: A 3D Standard of the Central Complex as a Platform for Neural Network Analysis.
The Locust Standard Brain: A 3D Standard of the Central Complex as a Platform for Neural Network Analysis.
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DOI：
10.3389/neuro.06.021.2009
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发表时间：
2009
影响因子：
3
通讯作者：
Homberg U
中科院分区：
文献类型：
--
作者：
El Jundi B;Heinze S;Lenschow C;Kurylas A;Rohlfing T;Homberg U
许多昆虫利用天空中的偏振光模式进行空间定位和导航。对沙漠蝗虫的偏振视觉系统进行了研究。为了创建一个用于偏振视觉通路解剖学研究的通用平台，Kurylas等人使用两种不同的标准化方法，即迭代形状平均（伊萨）程序和虚拟昆虫脑（VIB）协议，从10个男性大脑的共聚焦显微镜图像堆栈中生成了一个三维（3D）标准大脑。两种标准化方法的比较表明，VIB标准是理想的比较体积分析的neuropil，而伊萨标准是选择的方法来分析神经元的形态和连接。中枢复合体是蝗虫脑中极化信息处理的关键阶段。为了研究不同的中央复合体神经元之间的神经元连接，我们使用伊萨方法基于来自20个单独的中央复合体的脑切片生成了中央复合体和周围区域的更高分辨率的标准图谱。为了探索该图谱的有用性，两个中央复合体神经元，一个极化敏感的柱状神经元（CPU1a型）和一个在飞行过程中激活的切向神经元，巨型扇形（GFS）神经元，从大脑切片进行3D重建。为了检查GFS神经元是否是对CPU1a神经元的突触输入做出贡献的候选者，我们将两个神经元注册到标准化的中央复合体中。这两个神经元的可视化显示了中央体上部第二层的CPU1a和GFS神经元的潜在连接。
Many insects use the pattern of polarized light in the sky for spatial orientation and navigation. We have investigated the polarization vision system in the desert locust. To create a common platform for anatomical studies on polarization vision pathways, Kurylas et al. have generated a three-dimensional (3D) standard brain from confocal microscopy image stacks of 10 male brains, using two different standardization methods, the Iterative Shape Averaging (ISA) procedure and the Virtual Insect Brain (VIB) protocol. Comparison of both standardization methods showed that the VIB standard is ideal for comparative volume analysis of neuropils, whereas the ISA standard is the method of choice to analyze the morphology and connectivity of neurons. The central complex is a key processing stage for polarization information in the locust brain. To investigate neuronal connections between diverse central-complex neurons, we generated a higher-resolution standard atlas of the central complex and surrounding areas, using the ISA method based on brain sections from 20 individual central complexes. To explore the usefulness of this atlas, two central-complex neurons, a polarization-sensitive columnar neuron (type CPU1a) and a tangential neuron that is activated during flight, the giant fan-shaped (GFS) neuron, were reconstructed 3D from brain sections. To examine whether the GFS neuron is a candidate to contribute to synaptic input to the CPU1a neuron, we registered both neurons into the standardized central complex. Visualization of both neurons revealed a potential connection of the CPU1a and GFS neurons in layer II of the upper division of the central body.