Pseudoalteromonas arctica sp nov., an aerobic, psychrotolerant, marine bacterium isolated from Spitzbergen
Pseudoalteromonas arctica sp nov., an aerobic, psychrotolerant, marine bacterium isolated from Spitzbergen
复制标题
DOI：
10.1099/ijs.0.64963-0
复制
发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Antranikian, Garabed
中科院分区：
文献类型：
--
作者：
Al Khudary, Rami;Stoesser, Nele Isabelle;Antranikian, Garabed
从北极斯匹茨卑尔根的海水样本中分离出一种新的需氧耐冷海洋细菌，温度为4摄氏度。该菌株是一种极鞭毛革兰氏阴性菌，在含2-3% NaCl （w/v）的培养基中，以各种碳水化合物和有机酸为底物，在10-15℃、pH 7-8条件下生长最佳。主要脂肪酸成分包括16:0（占总脂肪酸的12.7%）、直链饱和脂肪酸甲酯（FAME）和16:1 omega 7c（40.2%）单不饱和FAME。系统发育分析表明，该分离株与假互变单胞菌（Pseudoalteromonas elyakovii KMM 162(T)）及其他假互变单胞菌属的亲缘关系较近，16S rRNA基因序列相似性达99%。新菌株DNA G + C含量为39 mol%。DNA-DNA杂交结果显示，与P. elyakovii KMM 162(T)的亲缘性为47.6%，与伪异单胞菌KMM 638(T)的亲缘性为44.2%，与黑质伪异单胞菌NCIMB 8614(T)的亲缘性为22.6%。基于系统发育和表型特征，该分离物代表了伪互变单胞菌属的一种新种，其名称为伪互变单胞菌北极；型应变为A 37-1-2(T) (=LMG 23753（T) =DSM 18437(T)）。
A novel aerobic, psychrotolerant marine bacterium was isolated at 4 degrees C from seawater samples collected from Spitzbergen in the Arctic. The strain was a polar-flagellated, Gram-negative bacterium that grew optimally at 10-15 degrees C and pH 7-8 in media containing 2-3% NaCl (w/v), using various carbohydrates and organic acids as substrates. The main fatty acid components included 16 : 0 (12.7 % of total fatty acids), straight-chain saturated fatty acid methyl ester (FAME) and 16: 1 omega 7c (40.2 %) monounsaturated FAME. Phylogenetic analysis revealed a close relationship (99 % 16S rRNA gene sequence similarity) between the novel isolate and Pseudoalteromonas elyakovii KMM 162(T) and some other species of the genus Pseudoalteromonas. The DNA G + C content of the novel strain was 39 mol%. DNA-DNA hybridization showed only 47.6 % DNA-DNA relatedness with P. elyakovii KMM 162(T), 44.2 % with Pseudoalteromonas distincta KMM 638(T) and 22.6 % with Pseudoalteromonas nigrifaciens NCIMB 8614(T). Based on phylogenetic and phenotypic characteristics, this isolate represents a novel species of the genus Pseudoalteromonas for which the name Pseudoalteromonas arctica is proposed; the type strain is A 37-1-2(T) (=LMG 23753(T) =DSM 18437(T)).