Large-scale evaluation of enzyme-linked immunospot assay and skin test for diagnosis of Mycobacterium tuberculosis infection against a gradient of exposure in The Gambia
Large-scale evaluation of enzyme-linked immunospot assay and skin test for diagnosis of Mycobacterium tuberculosis infection against a gradient of exposure in The Gambia
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DOI：
10.1086/382362
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发表时间：
2004-04-01
影响因子：
11.8
通讯作者：
McAdam, KPWJ
中科院分区：
文献类型：
--
作者：
Hill, PC;Brookes, RH;McAdam, KPWJ
结核分枝杆菌感染的纯化蛋白衍生物（PPD）皮试缺乏特异性。在冈比亚，我们通过酶联免疫斑点测定 (ELISPOT) 评估了 2 种更特异的结核分枝杆菌抗原（ESAT-6 和 CFP-10），并与通过 ELISPOT 和皮试进行的 PPD 进行了比较。在 130 例痰涂片阳性结核病病例的 735 名家庭接触者中，476 例（65%）通过 PPD ELISPOT 检测呈阳性，300 例（41%）通过 PPD 皮试检测呈阳性，218 例（30%）通过 ESAT-6/CFP-10 ELISPOT 检测呈阳性。只有 15 名 (2%) 的 ELISPOT 具有阳性 ESAT-6/CFP-10 结果和阴性 PPD 结果。随着结核分枝杆菌暴露的增加，PPD 皮试阳性/ESAT-6/CFP-10 ELISPOT 阴性的受试者百分比增加（P
The purified protein derivative (PPD) skin test for Mycobacterium tuberculosis infection lacks specificity. We assessed 2 more specific M. tuberculosis antigens (ESAT-6 and CFP-10) by enzyme-linked immunospot assay (ELISPOT) compared with PPD by ELISPOT and skin test in The Gambia. Of 735 household contacts of 130 sputum smear-positive tuberculosis cases, 476 (65%) tested positive by PPD ELISPOT, 300 (41%) tested positive by PPD skin test, and 218 (30%) tested positive by ESAT-6/CFP-10 ELISPOT. Only 15 (2%) had positive ESAT-6/CFP-10 results and negative PPD results by ELISPOT. With increasing M. tuberculosis exposure, the percentage of subjects who were PPD skin test positive/ESAT-6/CFP-10 ELISPOT negative increased (P