THE RETINAL-PIGMENT EPITHELIUM INDUCES FENESTRATION OF ENDOTHELIAL-CELLS INVIVO
THE RETINAL-PIGMENT EPITHELIUM INDUCES FENESTRATION OF ENDOTHELIAL-CELLS INVIVO
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DOI：
10.3109/02713689209033484
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发表时间：
1992-09-01
影响因子：
2
通讯作者：
HARTZ, MJ
中科院分区：
文献类型：
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作者：
BURNS, MS;HARTZ, MJ
啮齿动物感光细胞营养不良的特征在于视网膜血管向视网膜色素上皮（RPE）中的晚期向内生长，在视网膜色素上皮中它们增殖。这些血管中的一些发展脉络膜毛细血管（CC）的有孔表型。为了确定这些内皮细胞中窗孔的发育是否是内皮细胞被RPE包裹的持续时间的函数，我们对Long-Evans大鼠中乌拉坦诱导的感光细胞变性中的这些血管进行了超微结构形态测定研究。对20、24、40和56周龄的动物视网膜进行了研究，在20至56周龄之间，RPE内具有窗孔内皮细胞的血管轮廓的分数从10%增加到90%。每根血管的平均窗孔数在20 - 24周之间增加了约25倍，但此后稳定，尽管在56周时存在的血管数减少。40周时，RPE中可见大量变性的视网膜血管轮廓。这些事实支持RPE的存在诱导内皮细胞窗孔的想法，并且还表明在这些血管中发生了包括增殖和变性的复杂重塑过程。在这些啮齿动物模型中发生的新生血管的基本细胞生物学与增殖性糖尿病视网膜病变和年龄相关性黄斑变性之间的类比进行了讨论。
Rodent photoreceptor dystrophies are characterized by late stage ingrowth of retinal blood vessels into the retinal pigment epithelium (RPE) where they proliferate. Some of these vessels develop the fenestrated phenotype of the choriocapillaris (CC). To determine if development of fenestrae in these endothelial cells is a function of the duration of time the endothelial cell had been encapsulated by the RPE, we did an ultrastructural morphometric study of these vessels in urethane induced photoreceptor degeneration in Long-Evans rats. Retinas of animals aged 20, 24, 40 and 56 weeks were studied.The fraction of vessel profiles within the RPE that had fenestrated endothelial cells increased from 10% to 90% between 20 to 56 weeks. The average number of fenestrae per vessel increased approximately 25 fold between 20 and 24 weeks but stabilized after that, despite a decrease in the number of vessels present at 56 weeks. A large number of degenerated retinal vessel profiles were seen in the RPE at 40 weeks. These facts support the idea that the presence of the RPE induces endothelial cell fenestrae, and also show that a complex process of remodelling including proliferation and degeneration is occurring in these vessels. Analogies between the basic cell biology of neovascularization occuring in these rodent models and that of proliferative diabetic retinopathy and age-related macular degeneration are discussed.