Enhanced immune response after subcutaneous and oral immunization with biodegradable PLGA microspheres
Enhanced immune response after subcutaneous and oral immunization with biodegradable PLGA microspheres
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DOI：
10.1016/s0168-3659(98)00077-7
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发表时间：
1998-12-04
影响因子：
10.8
通讯作者：
Pedraz, JL
中科院分区：
文献类型：
--
作者：
Igartua, M;Hernández, RM;Pedraz, JL
采用双乳液/溶剂萃取法制备了以牛血清白蛋白（BSA）为模型抗原的PLGA微球，并对其进行了体外表征。在一系列体内研究中使用相同的微球来评估不同免疫方案皮下或口服接种后诱导的免疫反应。体内数据证实白蛋白的免疫原性不受封装过程的影响。皮下注射微球显示出的免疫反应（通过 ELISA 测定的血清 IgG 水平）统计上高于 BSA 溶液，即使注射剂量低 10 倍。发现微球的佐剂性与弗氏完全佐剂（FCA）相当，但与FCA相比，它们具有生物相容性，并且不会在注射部位引起任何不良反应。单次口服微球并不是诱导可重复反应的成功策略。因此，连续3天口服给予1和5μm的微球，获得的反应表明对于1μm的颗粒不需要使用加强剂量。这些结果表明，如果施用1μm颗粒，则有可能将免疫计划简化为初次免疫。 (C) 1998 Elsevier Science B.V. 保留所有权利。
PLGA microspheres containing bovine serum albumin (BSA) as a model antigen, were prepared by a double emulsion/solvent extraction method and their in vitro characterization was performed. The same microspheres were used in a series of in vivo studies to evaluate the immune response induced after subcutaneous or oral inoculation following different immunization protocols. The in vivo data confirm that the immunogenicity of the albumin is not affected by the encapsulation procedure. The subcutaneous administration of microspheres showed an immune response (serum IgG levels by ELISA) statistically above BSA solution, even when the dose administered was 10 times lower. The adjuvanticity of the microspheres was found to be comparable to that of Freund's complete adjuvant (FCA), but in contrast to FCA they are biocompatible and did not induce any adverse reaction at the site of injection. A single oral administration of the microspheres was not a successful strategy for the induction of a reproducible response. Therefore, microspheres of 1 and 5 mu m were orally administered on 3 consecutive days and the response obtained showed that the use of a boosting dose was not necessary for the 1 mu m particles. These results suggest the possibility of simplifying the immunization schedule to a primary immunization if 1 mu m particles are administered. (C) 1998 Elsevier Science B.V. All rights reserved.