AMINO-ACID METABOLISM OF PEA LEAVES - DIURNAL CHANGES AND AMINO-ACID SYNTHESIS FROM NITRATE-N-15
AMINO-ACID METABOLISM OF PEA LEAVES - DIURNAL CHANGES AND AMINO-ACID SYNTHESIS FROM NITRATE-N-15
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DOI：
10.1104/pp.59.5.915
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发表时间：
1977-01-01
期刊：
影响因子：
7.4
通讯作者：
JOY, KW
中科院分区：
文献类型：
--
作者：
BAUER, A;URQUHART, AA;JOY, KW
豌豆（Pisum sativum L.）植物中，氨基酸的水平有一个昼夜变化。在光照条件下，总氨基氮在前几个小时增加，然后趋于稳定;在黑暗条件下，有一个短暂的下降，然后逐渐恢复。天冬酰胺、高丝氨酸、丙氨酸和谷氨酰胺占这些变化的大部分。供应15 N-硝酸盐后，15 N掺入幼叶的各种成分中。15 N在氨中出现得最快，这是由于叶片中的还原，并且该过程主要发生在光照下。很大一部分的初级同化作用是通过谷氨酰胺的酰胺基团发生的，它被标记并迅速转化;谷氨酸和丙氨酸的标记也很快。天冬酰胺（酰胺组）很快被标记，并显示出相当大的周转。天冬氨酸、GABA和高丝氨酸的掺入和营业额较低。所有氨基酸的合成和周转在黑暗中以低速率继续进行。GABA是唯一一种在黑暗中比在光照下标记得更快的化合物。
In the young leaves of pea (Pisum sativum L.) plants, there was a diurnal variation in the levels of amino acids. In the light, total amino N increased for the 1st few hours, then stabilized; in the dark, there was a transient decrease followed by a gradual recovery. Asparagine, homoserine, alanine and glutamine accounted for much of these changes. The incorporation of 15N into various components of the young leaves was followed after supply of 15N-nitrate. 15N appeared most rapidly in ammonia, due to reduction in the leaf, and this process took place predominantly in the light. A large proportion of the primary assimilation took place through the amide group of glutamine, which became labeled and turned over rapidly; labeling of glutamic acid and alanine was also rapid. Asparagine (amide group) soon became labeled and showed considerable turnover. Slower incorporation and turnover were found for aspartic acid, GABA and homoserine. Synthesis and turnover of all of the amino acids continued at a low rate in the dark. GABA was the only compound found to label more rapidly in the dark than in the light.