Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos
Processing bodies and germ granules are distinct RNA granules that interact in C. elegans embryos
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DOI：
10.1016/j.ydbio.2008.07.008
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发表时间：
2008-11-01
影响因子：
2.7
通讯作者：
Seydoux, Geraldine
中科院分区：
文献类型：
--
作者：
Gallo, Christopher M.;Munro, Edwin;Seydoux, Geraldine
在体细胞中，未翻译的 mRNA 在称为加工体或 P 体的细胞质灶中积累。 P-体含有抑制翻译并刺激 mRNA 脱腺苷化、脱帽和衰变的复合物。最近，在生殖颗粒、生殖细胞特有的RNA颗粒中发现了某些P体蛋白。我们研究了秀丽隐杆线虫 P 体和胚芽颗粒之间可能的联系。我们鉴定出 PATR-1（酵母脱帽激活剂 Pat1p 的线虫同源物）作为线虫胚胎中 P 体的独特标记。我们发现 P 体作为核心颗粒从母体遗传，在体细胞和种系卵裂球中的成熟程度不同。在体细胞卵裂球中，P 体招募脱帽激活剂 LSM-1 和 LSM-3。这种招募需要 CCR4-NOT 去腺苷酶的 LET-711/Not1 亚基，并在空间和时间上与母体 mRNA 降解的开始相关。在种系卵裂球中，P-体保持为缺乏 LSM-1 和 LSM-3 的核心颗粒。 P-体与胚芽颗粒相互作用，但保持不同的动力学和成分。母体 mRNA nos-2 保留在胚芽颗粒中，但不保留在 P 体中。我们得出的结论是，P 体与生殖颗粒不同，代表第二类 RNA 颗粒，在体细胞和生殖细胞中表现不同。 (C) 2008 Elsevier Inc. 保留所有权利。
in somatic cells, untranslated mRNAs accumulate in cytoplasmic foci called processing bodies or P-bodies. P-bodies contain complexes that inhibit translation and stimulate mRNA deadenylation, decapping, and decay. Recently, certain P-body proteins have been found in germ granules, RNA granules specific to germ cells. We have investigated a possible connection between P-bodies and germ granules in Caenorhabditis elegans. We identify PATR-1, the C elegans homolog of the yeast decapping activator Pat1p, as a unique marker for P-bodies in C elegans embryos. We find that P-bodies are inherited maternally as core granules that mature differently in somatic and germline blastomeres. In somatic blastomeres, P-bodies recruit the decapping activators LSM-1 and LSM-3. This recruitment requires the LET-711/Not1 subunit of the CCR4-NOT deadenylase and correlates spatially and temporally with the onset of maternal mRNA degradation. in germline blastomeres, P-bodies are maintained as core granules lacking LSM-1 and LSM-3. P-bodies interact with germ granules, but maintain distinct dynamics and components. The maternal mRNA nos-2 is maintained in germ granules, but not in P-bodies. We conclude that P-bodies are distinct from germ granules, and represent a second class of RNA granules that behaves differently in somatic and germline cells. (C) 2008 Elsevier Inc. All rights reserved.