Analysis of the molecular basis of Saccharomyces cerevisiae mutant with high nucleic acid content by comparative transcriptomics
Analysis of the molecular basis of Saccharomyces cerevisiae mutant with high nucleic acid content by comparative transcriptomics
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比较转录组学分析酿酒酵母高核酸突变体的分子基础
DOI：
10.1016/j.foodres.2021.110188
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发表时间：
2021-02-09
影响因子：
8.1
通讯作者：
Xiao, Dongguang
中科院分区：
文献类型：
--
作者：
Guo, Xuewu;Zhao, Bin;Xiao, Dongguang
核糖核酸（RNA）及其降解产物是广泛应用于食品工业的重要功能成分。利用转录分析技术研究了高核酸含量酿酒酵母化学突变株BY23-195的核酸合成遗传机制。结果表明，核糖体生物合成、减数分裂、RNA转运、丝裂原活化蛋白激酶（MAPK）信号通路、色氨酸代谢、碳代谢和寿命调节通路与S.啤酒。选择14个最有希望的基因来评估单基因缺失或过表达对S.啤酒。与出发菌株BY23相比，突变株BY23-HXT 1、BY23-Delta GSP 2和BY23-Delta CTT 1的RNA含量分别提高了8.19%、11.60%和14.00%。HXT 1、GSP 2和CTT 1影响RNA含量的可能原因是通过调节细胞适应性。本研究首次报道了调控HXT 1、GSP 2和CTT 1的转录可以增加S.啤酒。本研究也为阐明S.酿酒酵母和新的策略，增加其RNA含量。
Ribonucleic acid (RNA) and its degradation products are important functional components widely used in the food industry. Transcription analysis was used to explore the genetic mechanism underlying nucleic acid synthesis in the chemical mutant Saccharomyces cerevisiae strain BY23-195 with high nucleic acid content. Results showed that ribosome biogenesis, meiosis, RNA transport, mitogen-activated protein kinase (MAPK) signaling pathway, tryptophan metabolism, carbon metabolism, and longevity regulating pathway are closely related to the high nucleic acid metabolism of S. cerevisiae. Fourteen most promising genes were selected to evaluate the effect of single-gene deletion or overexpression on the RNA synthesis of S. cerevisiae. Compared with the RNA content of the parent strain BY23, that of mutant strains BY23-HXT1, BY23-Delta GSP2 and BY23-Delta CTT1 increased by 8.19%, 11.60% and 14.00%, respectively. The possible reason why HXT1, GSP2, and CTT1 affect RNA content is by regulating cell fitness. This work was the first to report that regulating the transcription of HXT1, GSP2, and CTT1 could increase the RNA content of S. cerevisiae. This work also provides valuable knowledge on the genetic mechanism of high nucleic acid synthesis in S. cerevisiae and new strategies for increasing its RNA content.