Cloning of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from terpenoid antibiotic-producing Streptomyces strains
Cloning of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from terpenoid antibiotic-producing Streptomyces strains
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从产萜类抗生素的链霉菌菌株中克隆编码 3-羟基-3-甲基戊二酰辅酶 A 还原酶的基因
DOI：
10.1007/pl00008664
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
H. Seto
中科院分区：
文献类型：
--
作者：
T. Dairi;Y. Motohira;N. Itoh;T. Kuzuyama;S. Takahashi;H. Seto
摘要：我们通过筛选不产生萜烯素的突变体，从萜类抗生素（萜烯素）生产者灰孢链霉菌 MF730-N6 中分离出缺乏 3-羟基-3-甲基戊二酰辅酶 A (HMG-CoA) 还原酶活性的突变体，该菌株同时使用甲羟戊酸和非甲羟戊酸途径形成异戊烯基二磷酸。已知萜烯素是通过甲羟戊酸途径合成的。使用本研究中为菌株 MF730-N6 开发的自克隆系统，通过突变体的互补来克隆和鉴定编码 HMG-CoA 还原酶 (hmgg) 的基因。 HMG-CoA 还原酶的相应 hmgs 基因也从链霉菌属中克隆。 KO-3988，生产萜类抗生素呋喃喹啉。 hmgg和hmgs的序列分析表明，两个基因均编码353个氨基酸的多肽，彼此有84%的一致性。对蛋白质序列数据库的搜索显示，这两种基因产物也与来自多种其他生物体（包括链霉菌属 sp.）的 HMG-CoA 还原酶相似。 CL190（hmgg 与其 CL190 同源物有 89% 相同，hmgs 有 85% 相同）、海胆（40.3% 和 40.5%）、德国小蠊（37.6% 和 38.4%）以及喜树（39.7% 和 40.8%）。
Abstract We have isolated a mutant lacking 3-hydroxy-3-methylglutaryl CoA (HMG-CoA) reductase activity from a terpenoid antibiotic (terpentecin) producer, Streptomyces griseolosporeus MF730-N6, which uses both the mevalonate and nonmevalonate pathways for the formation of isopentenyl diphosphate, by screening terpentecin non-producing mutants. Terpentecin is known to be synthesized via the mevalonate pathway. The gene encoding HMG-CoA reductase (hmgg) was cloned and identified by complementation of the mutant, using a self-cloning system developed in this study for strain MF730-N6. The corresponding hmgs gene for HMG-CoA reductase was also cloned from Streptomyces sp. KO-3988, which produces the terpenoid antibiotic furaquinocin. Sequence analysis of hmgg and hmgs showed that both genes encode polypeptides of 353 amino acids which are 84% identical to each other. A search of protein sequence databases revealed that both gene products were also similar to HMG-CoA reductases from a variety of other organisms, including Streptomyces sp. CL190 (hmgg is 89% and hmgs 85% identical to its CL190 homolog), sea urchin (40.3 and 40.5%), German cockroach (37.6 and 38.4%), and Camptotheca acuminata (39.7 and 40.8%).
DOI：
10.1073/pnas.94.24.12857
发表时间：
1997-11-25
影响因子：
11.1
作者：
Sprenger, GA;Schorken, U;Sahm, H
通讯作者：
Sahm, H
叙利亚仓鼠 HMG-CoA 还原酶 cDNA 的核苷酸序列。
DOI：
10.1089/dna.1985.4.439
发表时间：
1985
期刊：
DNA (Mary Ann Liebert, Inc.)
影响因子：
--
作者：
Skalnik,DG;Simoni,RD
通讯作者：
Simoni,RD