Cytomegalovirus and human herpesvirus 6, but not human papillomavirus, are present in neonatal giant cell hepatitis and extrahepatic biliary atresia
Cytomegalovirus and human herpesvirus 6, but not human papillomavirus, are present in neonatal giant cell hepatitis and extrahepatic biliary atresia
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DOI：
10.1007/s100240010045
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发表时间：
2000-07-01
影响因子：
1.9
通讯作者：
Rogers, BB
中科院分区：
文献类型：
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作者：
Domiati-Saad, R;Dawson, DB;Rogers, BB
本研究的目的是证实人类乳头瘤病毒(HPV)与新生儿巨细胞性肝炎(GCH)和胆道闭锁(BA)有关的报道，并将这些研究扩大到包括巨细胞病毒(CMV)、爱泼斯坦-巴尔病毒(EBV)、人类疱疹病毒6(HHV6)和微小病毒B19(PVB19)。对19例GCH或BA患者和8例正常对照的肝组织冰冻切片进行聚合酶链式反应(PCR)分析。套式聚合酶链式反应检测HPV6型、16型、18型和33型，然后与普通探针进行P-32杂交。所有其他病毒均采用聚合酶链式反应，然后与地高辛标记的探针杂交。病例组和对照组均未检出HPV、EBV和PVB19。2例GCH和1例BA均为CMV阳性，对照组均为阴性。6例患者中有2例GCH、2例BA和2例正常对照检测到HHV6。我们的结论是，HPV与GCH或BA无关。在BA和GCH中检测到CMV证实了这种联系的其他报道。HHV6还需要进一步的研究，以确定在婴儿肝脏中进行阳性PCR检测的意义。
The purpose of our study was to confirm reports of an association of human papillomavirus (HPV) with neonatal giant cell hepatitis (GCH) and biliary atresia (BA), and to expand these studies to include cytomegalovirus (CMV), Epstein-Barr virus (EBV), human herpesvirus 6 (HHV6), and parvovirus B19 (PVB19). Frozen hepatic tissue was available for polymerase chain reaction (PCR) analysis in 19 cases of GCH or BA and 8 controls. Nested PCR to detect HPV types 6, 16, 18, and 33 was followed by P-32 hybridization with generic probes. PCR followed by hybridization with a digoxigenin-labeled probe was used for all other viruses. HPV, EBV, and PVB19 were not detected in cases or controls. Two cases of GCH and 1 case of BA were PCR positive for CMV; controls were negative. HHV6 was detected in 6 cases: 2 GCH, 2 BA, and 2 controls. We conclude that HPV is not associated with GCH or BA. Detection of CMV in BA and GCH confirms other reports of this association. HHV6 requires further study to determine the significance of a positive PCR test in the livers of infants.