Phosphatidylcholine is essential for efficient functioning of the mitochondrial glycerol-3-phosphate dehydrogenase Gut2 in Saccharomyces cerevisiae
Phosphatidylcholine is essential for efficient functioning of the mitochondrial glycerol-3-phosphate dehydrogenase Gut2 in Saccharomyces cerevisiae
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磷脂酰胆碱对于酿酒酵母线粒体 3-磷酸甘油脱氢酶 Gut2 的有效运作至关重要
DOI：
10.1080/09687860701210732
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发表时间：
2007
影响因子：
--
通讯作者：
A. D. de Kroon
中科院分区：
文献类型：
--
作者：
P. Rijken;B. Kruijff;A. D. de Kroon
线粒体甘油-3-磷酸脱氢酶Gut2先前被证明优先标记为光活化磷脂酰胆碱（PC），指出了这些分子之间的功能关系。在本研究中，我们利用降低PC含量的PC生物合成突变体分析了Gut2功能是否依赖于酵母细胞的PC含量。研究发现，PC消耗减少了对甘油的生长，增加了甘油的排泄，两者都表明PC是Gut2体内最佳功能所必需的。使用几种体外方法研究了Gut2功能对细胞PC含量的依赖性质。这些实验结果表明，体内观察到的影响不太可能是由于降低PC水平后细胞Gut2含量、分离线粒体中Gut2的特定活性或Gut2的膜关联的变化。体内效应更可能是细胞环境中PC消耗诱导的Gut2功能变化的间接结果，而在体外系统中并未表现出来。
Gut2, the mitochondrial glycerol-3-phosphate dehydrogenase, was previously shown to become preferentially labelled with photoactivatable phosphatidylcholine (PC), pointing to a functional relation between these molecules. In the present study we analyzed whether Gut2 functioning depends on the PC content of yeast cells, using PC biosynthetic mutants in which the PC content was lowered. PC depletion was found to reduce growth on glycerol and to increase glycerol excretion, both indicating that PC is needed for optimal Gut2 functioning in vivo. Using several in vitro approaches the nature of the dependence of Gut2 functioning on cellular PC contents was investigated. The results of these experiments suggest that it is unlikely that the effects observed in vivo are due to changes in cellular Gut2 content, in specific activity of Gut2 in isolated mitochondria, or in the membrane association of Gut2, upon lowering the PC level. The in vivo effects are more likely an indirect result of PC depletion-induced changes in the cellular context in which Gut2 functions, that are not manifested in the in vitro systems used.
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DOI：
10.1073/pnas.89.5.1671
发表时间：
1992-03-01
影响因子：
11.1
作者：
NITSCH, RM;BLUSZTAJN, JK;WURTMAN, RJ
通讯作者：
WURTMAN, RJ
酿酒酵母 opi3 基因突变：对磷脂甲基化、生长和肌醇合成跨途径调节的影响。
影响因子：
3.3
作者：
McGraw,P;Henry,SA
通讯作者：
Henry,SA
影响因子：
3.3
作者：
Boumann, HA;Gubbens, J;de Kroon, AIPM
通讯作者：
de Kroon, AIPM
酿酒酵母 cho2 突变体缺乏磷脂甲基化和肌醇合成的跨途径调节。
影响因子：
3.3
作者：
Summers,EF;Letts,VA;McGraw,P;Henry,SA
通讯作者：
Henry,SA
影响因子：
5.6
作者：
EISENBERG, D;SCHWARZ, E;WALL, R
通讯作者：
WALL, R