H. pylori eradication did not improve dysregulation of specific oncogenic miRNAs in intestinal metaplastic glands
H. pylori eradication did not improve dysregulation of specific oncogenic miRNAs in intestinal metaplastic glands
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DOI：
10.1007/s00535-012-0562-7
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发表时间：
2012-09-01
影响因子：
6.3
通讯作者：
Haruma, Ken
中科院分区：
文献类型：
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作者：
Shiotani, Akiko;Uedo, Noriya;Haruma, Ken
许多microRNAs （miRNAs）在幽门螺杆菌感染的胃粘膜和胃癌组织中差异表达。我们的目的是比较幽门螺杆菌根除对胃癌高危人群胃粘膜mirna的影响。近期有早期胃癌内镜切除术史的患者和性别和年龄匹配的非癌症对照组被纳入研究。在幽门螺杆菌根除前和一年后，通过胃粘膜活检标本和胃小弯和大弯胃腺显微解剖来检测21个miRNAs的表达。20名患者和14名对照者入组。致癌mirna （miR-17/92和miR-106b-93-25簇，miR-21， miR-194和miR-196）在胃癌组胃粘膜中的表达明显高于对照组。幽门螺杆菌根除只导致对照组中致癌mirna的表达显著下降，而两组中miR-223表达降低，let-7d表达升高。miR-196仅在肠化生腺体中表达。无论是否根除幽门螺杆菌，致癌性mirna在肠化生腺中的表达均显著高于非肠化生腺。在两组中，根除幽门螺杆菌均未显著改变肠化生腺中任何miRNA的表达。特定mirna的失调存在于幽门螺杆菌诱导的主体胃炎中。幽门螺杆菌根除改善了miRNA失调，但在胃癌高危人群的肠化脓腺和胃粘膜中没有改善。
Many microRNAs (miRNAs) are differentially expressed in Helicobacter pylori-infected gastric mucosa and in gastric cancer tissue.We aimed to compare the effect of H. pylori eradication on gastric mucosal miRNAs in subjects in a high-risk group for gastric cancer compared to controls.Patients with a recent history of endoscopic resection for early gastric cancer and sex- and age-matched non-cancer controls were enrolled. The expression of 21 miRNAs was examined using gastric mucosal biopsy specimens and microdissected gastric glands from the lesser and greater curvatures of the gastric corpus both before and one year after H. pylori eradication.Twenty patients and 14 controls were enrolled. The expression of oncogenic miRNAs (miR-17/92 and the miR-106b-93-25 cluster, miR-21, miR-194, and miR-196) was significantly higher in the gastric mucosa of the cancer group than in the controls. H. pylori eradication resulted in a significant fall in the expression of oncogenic miRNAs only in the controls, whereas miR-223 expression was decreased and let-7d expression was increased in both groups. miR-196 was expressed only in intestinal metaplastic glands. The expression of oncogenic miRNAs was significantly higher in the intestinal metaplastic glands than in the non-intestinal metaplastic glands irrespective of H. pylori eradication. In neither group did H. pylori eradication significantly change any miRNA expression in the intestinal metaplastic glands.Dysregulation of specific miRNAs is present in H. pylori-induced corpus gastritis. H. pylori eradication improved miRNA dysregulation, but not in intestinal metaplastic glands or in the gastric mucosa of patients in a high-risk group for gastric cancer.