Detection of T-2 toxin by an improved radioimmunoassay
Detection of T-2 toxin by an improved radioimmunoassay
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通过改进的放射免疫分析法检测 T-2 毒素
DOI：
10.1128/aem.45.2.640-643.1983
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发表时间：
1983
影响因子：
4.4
通讯作者：
F. Chu
中科院分区：
文献类型：
--
作者：
P. Fontelo;J. Beheler;D. Bunner;F. Chu
用改良的放射免疫法分析血清、尿液和生理盐水中的T-2毒素。样品直接加入试管，不需要提取步骤。抗体与配体的最佳反应时间为1h。白蛋白包裹的木炭用于分离结合和自由放射性。用60%高氯酸和30%过氧化氢的湿式氧化过程纠正了溶血样品发生的猝灭。较短的孵育时间导致了一种不到6小时即可完成的检测。抗体的平均亲和常数(Km)为1.75×10(10)L/mol。灵敏度为1 ng/次或10 ng/ml。在所检测的其他三氯乙烯中，只有H-T-2有显著的交叉反应(10.3%)。
T-2 toxin in serum, urine, and saline was analyzed by a modified radioimmunoassay procedure. The specimens were added directly to the assay tubes without extraction steps. The reaction between antibody and ligands was optimal at 1 h. Albumin-coated charcoal was used to separate bound from free radioactivity. Quenching, which occurred with hemolyzed specimens, was corrected by a wet oxidation process with 60% perchloric acid and 30% hydrogen peroxide. The shorter incubation times resulted in an assay that takes less than 6 h to complete. The average affinity constant of the antibody (Km) was 1.75 X 10(10) liters/mol. The sensitivity was 1 ng per assay or 10 ng/ml. Among the other trichothecenes tested, only H-T-2 cross-reacted significantly (10.3%).