Amplification of 19q13.1-q13.2 sequences in ovarian cancer - G-band, FISH, and molecular studies
Amplification of 19q13.1-q13.2 sequences in ovarian cancer - G-band, FISH, and molecular studies
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DOI：
10.1016/0165-4608(95)00248-0
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发表时间：
1996-03-01
影响因子：
--
通讯作者：
Taetle, R
中科院分区：
文献类型：
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作者：
Thompson, FH;Nelson, MA;Taetle, R
在这项卵巢癌的研究中，我们通过使用19号染色体油漆和microFISH探针进行FISH，并通过G显带鉴定19号染色体异常和不异常的患者样本，扩展了先前的发现。der（19）的核型解释得到了证实，而在某些情况下，19 WCP的FISH也检测到了另外19个易位。卵巢癌细胞系的类似FISH研究发现，即使在广泛的体外培养后，19号染色体也异常。采用显微切割技术制备了2例hsr（19）患者的MicroFISH探针，分别定位于19q13.2和19q13.1-.2。用这些microFISH探针单独或与19 WCP探针组合对4个患者样本和7个细胞系进行FISH，结果显示65%的19号染色体结构异常包含19q13.1-q13.2序列，有时为大的hsrs。卵巢癌细胞系显示AKT 2推定癌基因的扩增和过表达，但在该染色体区域没有ERCC-2 DNA修复基因。除AKT 2外，19q13.1-q13.2区域中其他尚未鉴定的基因的扩增和过表达可能有助于卵巢癌的发病或进展。
In this study of ovarian carcinoma, we extended previous findings by performing FISH using chromosome 19 paint and microFISH probes and patient samples with and without abnormalities of chromosome 19 identified by G-banding. Karyotype interpretations of der(19) were confirmed, while additional 19 translocations were also detected by FISH with 19WCP in some cases. Similar FISH studies of ovarian carcinoma cell lines found chromosome 19 abnormalities even after extensive in vitro culture. MicroFISH probes were generated by chromosome microdissection from two cases with hsr(l9) and mapped to 19q13.2 and 19q13.1-.2, respectively. FISH with these microFISH probes alone or in combination with a 19 WCP probe to four patient samples and seven cell lines showed that 65% of chromosome 19 structural abnormalities contained 19q13.1-q13.2 sequences, sometimes as large hsrs. Ovarian cancer cell lines showed amplification and overexpression of the AKT2 putative oncogene, but not the ERCC-2 DNA repair gene in this chromosomal region. In addition to AKT2, amplification and overexpression of other yet-unidentified genes in the 19q13.1-q13.2 region may contribute to ovarian carcinoma pathogenesis or progression.