Identification of collagen as a new fish allergen
Identification of collagen as a new fish allergen
复制标题
DOI：
10.1271/bbb.65.285
复制
发表时间：
2001-02-01
影响因子：
1.6
通讯作者：
Shiomi, K
中科院分区：
文献类型：
--
作者：
Hamada, Y;Nagashima, Y;Shiomi, K
本研究旨在鉴定在鱼类中检测到的高分子量过敏原。通过ELISA分析从大眼金枪鱼肌肉中制备的五种蛋白组分，结果表明，肌基质蛋白组分中含有高分子量过敏原。根据肌基质蛋白组分的SDS-PAGE、免疫印迹和氨基酸分析结果，判断高分子量变应原为胶原蛋白。发现所用的八个患者血清中的五个与大眼金枪鱼胶原蛋白反应。在竞争性ELISA抑制实验中，大眼金枪鱼胶原蛋白几乎完全抑制了IgE反应性的加热提取物从五种鱼，这表明胶原蛋白是常见的过敏，无论鱼类。然而，没有观察到来自鱼和其他动物的胶原蛋白之间的抗原交叉反应。
This study was intended to identify a high molecular weight allergen that had been detected in fish. Analyses by ELISA of five protein fractions prepared from bigeye tuna muscle showed that the high molecular weight allergen was contained in the myostromal protein fraction. Based on the results of SDS-PAGE, immunoblotting and amino acid analysis of the myostromal protein fraction, the high molecular weight allergen was judged to be collagen. Five of the eight patient sera used were found to react to the bigeye tuna collagen. In competitive ELISA inhibition experiments, the bigeye tuna collagen almost completely inhibited the IgE reactivity to the heated extracts from five species of fish, suggesting that collagen is commonly allergic regardless of fish species. However, no antigenic cross-reactivity was observed between collagens from fish and other animals.