Critical steps in the production of polyclonal and monoclonal antibodies evaluation and recommendations
Critical steps in the production of polyclonal and monoclonal antibodies evaluation and recommendations
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DOI：
10.1093/ilar.46.3.269
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发表时间：
2005-01-01
期刊：
影响因子：
2.5
通讯作者：
Hendriksen, CFM
中科院分区：
文献类型：
--
作者：
Leenaars, M;Hendriksen, CFM
抗体在实验室和临床都是很有价值的工具。抗体包括由单个B淋巴细胞克隆分泌的抗体。称为单克隆抗体，而由多种B淋巴细胞克隆的混合物产生的抗体，称为多克隆抗体。这两种产品已成为基础免疫学研究、免疫组织化学、诊断测试和疫苗质量控制的重要工具。抗体的生产需要大量的动物，而这些动物要经受许多侵入性的程序，如抗原注射和血液采集。然而，通过精心设计免疫方案并优化免疫反应，可以在获得最佳免疫反应的同时最大限度地减少动物的疼痛和痛苦。在这篇文章中，描述了生产多克隆和单克隆抗体的关键步骤，特别是包括动物种类和年龄的选择，注射方案和腹水的挖掘。提出了优化免疫反应的建议。
Antibodies are valuable tools in the laboratory and clinic. Antibodies include those secreted by a single clone of B lymphocytes. termed monoclonal antibodies, and those produced by a mixture of various B lymphocyte clones, termed polyclonal antibodies. Both products have become essential instruments in fundamental immunological research, immunohistochemistry, diagnostic testing, and vaccine quality control. Antibody production requires a substantial number of animals, and the animals are subjected to a number of invasive procedures such as antigen injection and blood collection. However, by carefully designing an immunization protocol and by optimizing the immunization response, it is possible to minimize animals' pain and distress while obtaining optimal immune responses. In this article, the critical steps in the production of polyclonal and monoclonal antibodies are described, specifically including selection of the animal species and its age, injection protocol, and ascites tapping. Recommendations are provided for optimizing the immunization response.