Systemic Effects of Radiotherapy and Concurrent Chemo-Radiotherapy in Head and Neck Cancer Patients-Comparison of Serum Metabolome Profiles
Systemic Effects of Radiotherapy and Concurrent Chemo-Radiotherapy in Head and Neck Cancer Patients-Comparison of Serum Metabolome Profiles
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DOI：
10.3390/metabo10020060
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发表时间：
2020-02-01
期刊：
影响因子：
4.1
通讯作者：
Widlak, Piotr
中科院分区：
文献类型：
--
作者：
Jelonek, Karol;Krzywon, Aleksandra;Widlak, Piotr
抗癌治疗诱导可以在生物流体水平检测到的全身性分子变化。了解这些治疗如何影响人体代谢对于预测个体反应和调整个性化治疗至关重要。在这里，我们的目的是比较代谢物的配置文件在头颈部癌症患者的血清治疗同步放化疗，放疗，或诱导化疗。血清样品进行了分析，通过有针对性的定量方法，使用组合直接流动注射和液相色谱串联质谱法，允许同时定量的149种代谢物。有45种代谢产物的水平在治疗前和治疗内或治疗后血清样品之间发生了显著变化，包括38种磷脂。同步放化疗比单纯放疗起效更快、效果更强。另一方面，单独化疗并没有导致显著的变化。总磷脂水平降低是在处理的第一步期间观察到的最明显的效应。这对应于患者的体重减轻，但在个体患者中未观察到两个参数之间的相关性。我们的结论是，不同的分子变化，在血清代谢组学水平上测量不同的治疗方式。
Anticancer treatment induces systemic molecular changes that could be detected at the level of biofluids. Understanding how human metabolism is influenced by these treatments is crucial to predict the individual response and adjust personalized therapies. Here, we aimed to compare profiles of metabolites in serum of head and neck cancer patients treated with concurrent chemo-radiotherapy, radiotherapy alone, or induction chemotherapy. Serum samples were analyzed by a targeted quantitative approach using combined direct flow injection and liquid chromatography coupled to tandem mass spectrometry, which allowed simultaneous quantification of 149 metabolites. There were 45 metabolites whose levels were significantly changed between pretreatment and within- or post-treatment serum samples, including 38 phospholipids. Concurrent chemo-radiotherapy induced faster and stronger effects than radiotherapy alone. On the other hand, chemotherapy alone did not result in significant changes. The decreased level of total phospholipids was the most apparent effect observed during the first step of the treatment. This corresponded to the loss of patients' body mass, yet no correlation between both parameters was observed for individual patients. We concluded that different molecular changes were measured at the level of serum metabolome in response to different treatment modalities.