NEUTROPHIL INFLUX INTO GUINEA-PIG AIRWAY LUMEN DURING CHOLINERGIC AND NONCHOLINERGIC BRONCHOCONSTRICTION
NEUTROPHIL INFLUX INTO GUINEA-PIG AIRWAY LUMEN DURING CHOLINERGIC AND NONCHOLINERGIC BRONCHOCONSTRICTION
复制标题
DOI：
10.1111/j.1748-1716.1992.tb09272.x
复制
发表时间：
1992-01-01
期刊：
影响因子：
--
通讯作者：
CHUNG, KF
中科院分区：
文献类型：
--
作者：
LOTVALL, J;RABE, K;CHUNG, KF
感觉神经的激活会使中性粒细胞粘附在气管支气管微血管上。本研究的目的是调查这种依从性是否会导致中性粒细胞流入气道管腔。为此，我们研究了麻醉和气管造口豚鼠迷走神经刺激20分钟（1 Hz, 5 ms, 5 V）对肺阻力和支气管肺泡灌洗细胞图像的影响。将任何变化与静脉输注甲胆碱的变化进行比较，产生类似的肺阻力变化。由于高压通气可能造成肺损伤，我们还研究了通过体外电阻通气的影响，产生类似的变化。经肺压（45 +/- 2 cmH2O）作为迷走神经刺激（42 +/- 4 cmH2O）。迷走神经刺激、注入甲胆碱或体外电阻器均未使灌洗后恢复的细胞总数增加。然而，与假刺激相比，迷走神经刺激和甲胆碱注入均显著增加了灌洗液中中性粒细胞的相对数量（分别为21 +/- 11%，13 +/- 4%和4 +/- 1%），但体外电阻没有影响（4 +/- 2%）。我们的数据表明，长时间的支气管收缩本身可能诱导中性粒细胞流入豚鼠的气道管腔。
Sensory nerve activation will produce adherence of neutrophils to tracheobronchial microvessels. The aim of the present study was to investigate whether this adherence would lead to an influx of neutrophils into the airway lumen. To do this, we studied the effects of 20 minutes of vagal stimulation (1 Hz, 5 ms, 5 V) in anaesthetized and tracheostomized guinea-pigs on both lung resistance, and the cell picture in bronchoalveolar lavage. Any changes were compared to those of intravenous methacholine influsion, producing similar changes in lung resistance. Since high pressure ventilation could produce lung damage, we also studied the effects of ventilation through an extracorporeal resistor, producing a similar change in. transpulmonary pressure (45 +/- 2 cmH2O) as vagal stimulation (42 +/- 4 cmH2O). The total number of cells recovered in the lavage was not increased by vagal stimulation, methacholine influsion or the extracorporeal resistor. However, both vagal stimulation and methacholine influsion significantly increased the relative number of neutrophils in the lavage compared to sham stimulated animals (21 +/- 11%, 13 +/- 4% and 4 +/- 1% respectively), but the extracorporeal resistor had no effect (4 +/- 2%). Our data suggests that prolonged bronchoconstriction per se may induce an influx of neutrophils into the airway lumen of the guinea-pig.