Establishment and characterization of nine new head and neck cancer cell lines
Establishment and characterization of nine new head and neck cancer cell lines
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DOI：
10.3109/00016489709113477
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发表时间：
1997-09-01
影响因子：
1.4
通讯作者：
Carey, TE
中科院分区：
文献类型：
--
作者：
Kim, SY;Chu, KC;Carey, TE
我们从头颈癌患者中建立了新的细胞系，用于研究在牙山医疗中心治疗的9名原发性或转移性癌症患者的粘附分子和细胞行为。新鲜肿瘤组织的外植体培养物用于开发新的永久性肿瘤细胞系。在裸鼠中测试细胞系的肿瘤形成和组织学。流式细胞术和间接免疫荧光法用于评估DNA含量和α 6、β 4和β 1整合素亚基和细胞间粘附分子1（ICAM-1）的表达。使用相差显微镜评估体外生长模式和对塑料的粘附。AMC-HN-1至-8来自鳞状细胞癌患者。AMC-HN-9来自腮腺未分化癌。我们测试的8个细胞系产生了与原始肿瘤的组织学相同的裸鼠肿瘤。AMC-HN-1、-2、-5和-9具有上皮样或梭形细胞形态，细胞间和细胞间基质粘附性差。AMC-HN-3、-4、-7和-8作为粘附上皮样单层生长。AMC-HN-6具有多层分层。4个系为近二倍体，4个系为超二倍体，1个系为亚二倍体。只有三个表达ICAM-1。所有细胞系均表达α 6、β 4和β 1整联蛋白亚基，但表达程度不同。四种，AMC-HN-1、-2、-5和-6以低水平表达β 4整联蛋白，AMC-HN-3、-4、-7和-9具有中等β 4表达，并且AMC-HN-8具有极高的β 4表达。AMC-HN细胞系是头颈癌生物学研究的代表性体外模型。我们的初步结果表明，整合素表达和细胞粘附在体外的密切关系。
We established new cell lines from head and neck cancer patients for studies of adhesion molecules and cellular behavior in nine patients with primary or metastatic cancer treated at the Asan Medical Center. Explant cultures of fresh tumor tissue were used to develop new permanent tumor cell lines. Lines were tested for tumor formation and histology in nude mice. Flow cytometry and indirect immunofluorescence were used to assess DNA content and expression of the alpha 6, beta 4, and beta 1 integrin subunits and the intercellular adhesion molecule 1 (ICAM-1). In vitro growth patterns and adhesion to plastic were assessed using phase contrast microscopy. AMC-HN-1 to -8 were derived from patients with squamous cell carcinoma. AMC-HN-9 was from an undifferentiated carcinoma of the parotid gland. The 8 lines we tested produced nude mouse tumors that are identical to the histology of the original tumors. AMC-HN-1, -2, -5, and -9 have epithelioid or spindle cell morphology with poor cell-to-cell and cell-to-substrate adhesiveness. AMC-HN-3, -4, -7, and -8 grow as adherent epithelioid monolayers. AMC-HN-6 exhibits multilayer stratification. Four lines are near diploid, 4 are hyperdiploid and 1 is hypodiploid. Only three express ICAM-1. All lines express the alpha 6, beta 4, and beta 1 integrin subunits but to different extent. Four, AMC-HN-1, -2, -5, and -6, express the beta 4 integrin at low levels, AMC-HN-3, -4, -7, and -9, have intermediate beta 4 expression, and AMC-HN-8 has extremely high beta 4 expression. The AMC-HN cell lines are representative in vitro models for the study of head and neck cancer biology. Our preliminary results indicate a close relationship between integrin expression and cell adhesion in vitro.