The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2
The expression of Lin28B was co-regulated by H3K4me2 and Wnt5a/β-catenin/TCF7L2
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Lin28B 的表达由 H3K4me2 和 Wnt5a/β-连环蛋白/TCF7L2 共同调节
DOI：
10.1016/s2095-3119(20)63441-4
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发表时间：
2020-12-01
影响因子：
4.8
通讯作者：
Li Bi-chun
中科院分区：
文献类型：
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作者：
Zhang Ya-ni;Hu Cai;Li Bi-chun
Lin 28 A和Lin 28 B是同源的RNA结合蛋白，参与原始生殖细胞的发育。Lin 28 A的表达和调控机制已被充分证明，但Lin 28 B的信息有限。本研究克隆了Lin 28 B启动子片段，构建了pEGFP-pLin 28 B载体。转染DF-1鸡成纤维细胞，检测绿色荧光蛋白（GFP）的表达。进一步采用染色体步移法克隆Lin 28 B启动子不同长度的片段，并将其连接到PGL 3-Basic载体中，转染DF-1细胞。双荧光素酶报告基因检测结果表明，Lin 28 B启动子的核心序列在-1431 ~-1034bp之间。通过生物信息学分析，确定了转录因子TCF 7 L2的结合位点。Lin 28 B启动子活性下调（P
Lin28A and Lin28B are homologous RNA-binding proteins that participate in the development of primordial germ cells. The mechanisms underlying expression and regulation of Lin28A have been well documented, but such information for Lin28B is limited. In this study, a fragment of the Lin28B promoter was cloned, the pEGFP-pLin28B vector was constructed. DF-1 chicken fibroblasts were transfected and the expression of green fluorescent protein (GFP) was measured. Furtherly, Lin28B promoter of different lengths fragments was cloned using the chromosome-walking method and the fragments were ligated into the PGL3-Basic vector, and transfected into DF-1 cells. Results of dual-luciferase reporter assay showed that the core of the Lin28B promoter was included in the sequence from -1 431 to -1 034 bp. The binding sites of the transcription factor TCF7L2 was showed within this sequence by bioinformatics analysis. The promoter activity of Lin28B was downregulated (P