Spatial segregation of mRNA encoding myelin-specific proteins.
Spatial segregation of mRNA encoding myelin-specific proteins.
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编码髓磷脂特异性蛋白的 mRNA 的空间分离。
DOI：
10.1073/pnas.84.21.7773
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发表时间：
1987
影响因子：
11.1
通讯作者：
Griffin,J
中科院分区：
文献类型：
--
作者：
Trapp,BD;Moench,T;Pulley,M;Barbosa,E;Tennekoon,G;Griffin,J
通过原位杂交，研究了在发育中的大鼠神经系统组织切片中编码三种髓鞘特异性蛋白（髓鞘碱性蛋白（MBP）、蛋白脂质蛋白（PLP）和Po蛋白）的mrna的细胞和亚细胞分布。这些mrna的发育外观与它们编码的蛋白质的外观密切相关，这是由免疫细胞化学确定的。编码外源性膜蛋白（MBP）的mRNA在髓鞘形成初期集中在少突胶质细胞和雪旺细胞核周围；随着髓鞘形成的进行，MBP mRNA在髓鞘纤维上弥漫性分布。相比之下，编码内在膜蛋白PLP和Po的mrna在髓鞘形成的所有阶段仍然集中在少突胶质细胞（PLP）和雪旺细胞（Po）细胞核周围。这些结果证实，髓鞘细胞在空间上分离某些髓鞘特异性mrna。髓鞘节间细胞质区域内MBP mRNA的存在表明，髓鞘形成过程中的蛋白质分选涉及mRNA转运到特定的亚细胞位点。
The cellular and subcellular distributions of mRNAs encoding three myelin-specific proteins--myelin basic protein (MBP), proteolipid protein (PLP), and Po protein--were studied in tissue sections of developing rat nervous systems by in situ hybridization. The developmental appearance of these mRNAs closely paralleled the appearance of the proteins they encode as determined by immunocytochemistry. mRNA encoding the extrinsic membrane protein, MBP, was concentrated around oligodendrocyte and Schwann cell nuclei during initial stages of myelination; as myelination proceeded, MBP mRNA became distributed diffusely over myelinated fibers. In contrast, mRNAs encoding the intrinsic membrane proteins, PLP and Po, remained concentrated around oligodendrocyte (PLP) and Schwann cell (Po) nuclei at all stages of myelination. These results establish that myelinating cells spatially segregate certain myelin-specific mRNAs. The presence of MBP mRNA within the cytoplasmic domains of myelin internodes indicates that protein sorting during myelination involves transportation of mRNA to specific subcellular sites.