UVR8 in Arabidopsis thaliana regulates multiple aspects of cellular differentiation during leaf development in response to ultraviolet B radiation
UVR8 in Arabidopsis thaliana regulates multiple aspects of cellular differentiation during leaf development in response to ultraviolet B radiation
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DOI：
10.1111/j.1469-8137.2009.02855.x
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发表时间：
2009-01-01
期刊：
影响因子：
9.4
通讯作者：
Paul, Nigel D.
中科院分区：
文献类型：
--
作者：
Wargent, Jason J.;Gegas, Vasilis C.;Paul, Nigel D.
P>对于紫外线 B (UV-B) 波长的特异性反应，无论是在初始信号传导还是对形态发生的影响方面，人们仍然知之甚少。拟南芥抗紫外线基因座8 (UVR8) 是唯一已知的UV-B 特异性信号成分，但UVR8 在叶片形态发生中的作用尚不清楚。对UVR8 在一系列补充UV-B 剂量下对叶片形态发生的调节作用进行了表征，揭示了对UV 照射的UVR8 依赖性和独立反应。对UV-B 响应的表皮细胞分裂的抑制在很大程度上独立于UVR8。然而，与uvr8突变体相比，野生型植物在UV-B照射下的整体叶片生长得到增强，因为野生型植物中细胞面积依赖于UVR8的补偿性增加。 UVR8 也是调节内多倍体响应 UV-B 所必需的，并且在 UV-B 存在的情况下，uvr8 突变体的气孔密度也比野生型更低，表明 UVR8 在其他发育事件中具有调节作用。我们的研究结果表明，除了调节紫外线保护基因表达反应外，UVR8 还参与控制叶片生长和形态发生。这项工作扩展了我们对 UV-B 响应如何在全植物水平上协调的理解。New Phytological (2009) 183: 315-326doi: 10.1111/j.1469-8137.2009.02855.x。
P>Responses specific to ultraviolet B (UV-B) wavelengths are still poorly understood, both in terms of initial signalling and effects on morphogenesis. Arabidopsis thaliana UV RESISTANCE LOCUS8 (UVR8) is the only known UV-B specific signalling component, but the role of UVR8 in leaf morphogenesis is unknown.The regulatory effects of UVR8 on leaf morphogenesis at a range of supplementary UV-B doses were characterized, revealing both UVR8-dependent and independent responses to UV irradiation.Inhibition of epidermal cell division in response to UV-B is largely independent of UVR8. However, overall leaf growth under UV-B irradiation in wild-type plants is enhanced compared with a uvr8 mutant because of a UVR8-dependent compensatory increase of cell area in wild-type plants. UVR8 was also required for the regulation of endopolyploidy in response to UV-B, and the uvr8 mutant also has a lower density of stomata than the wild type in the presence of UV-B, indicating that UVR8 has a regulatory role in other developmental events.Our findings show that, in addition to regulating UV-protective gene expression responses, UVR8 is involved in controlling aspects of leaf growth and morphogenesis. This work extends our understanding of how UV-B response is orchestrated at the whole-plant level.New Phytologist (2009) 183: 315-326doi: 10.1111/j.1469-8137.2009.02855.x.