THE GLYCOPROTEIN OF INFLUENZA-C VIRUS IS THE HEMAGGLUTININ, ESTERASE AND FUSION FACTOR
THE GLYCOPROTEIN OF INFLUENZA-C VIRUS IS THE HEMAGGLUTININ, ESTERASE AND FUSION FACTOR
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DOI：
10.1099/0022-1317-69-4-839
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发表时间：
1988-04-01
影响因子：
3.8
通讯作者：
KLENK, HD
中科院分区：
文献类型：
--
作者：
HERRLER, G;DURKOP, I;KLENK, HD
在丙型流感病毒的生物学活性中，血凝、受体失活和融合，只有后者与其表面糖蛋白（gp）最终相关。我们通过辛基葡萄糖苷处理C型流感病毒粒子，然后离心到蔗糖梯度中来纯化gp。有证据表明，gp也是C型流感病毒的受体破坏酶，其特征是神经氨酸9-O-乙酰酯酶：（i）它使鸡红细胞上的C型流感病毒受体失活;（ii）它具有乙酰酯酶活性，如牛下颌骨粘蛋白释放乙酸所示;（iii）针对gp的单克隆抗体抑制C型流感病毒的乙酰酯酶活性。虽然纯化的gp不能凝集鸡红细胞，但它能阻断病毒引起的血细胞凝集。这一发现以及单克隆抗gp抗体的血凝抑制活性表明，gp也负责丙型流感病毒的血凝活性。因此，由于丙型流感病毒糖蛋白是唯一具有三种不同活性的粘病毒糖蛋白，因此我们建议命名HEF，以描述其作为血凝（H）、酯酶（E）和融合因子（F）的功能。
Of the biological activities of influenza C virus, haemagglutination, receptor inactivation and fusion, only the latter has been conclusively correlated with its surface glycoprotein (gp). We have purified the gp by octylglucoside treatment of influenza C virions followed by centrifugation into a sucrose gradient. Evidence was obtained that gp also represents the receptor-destroying enzyme of influenza C virus, which has been characterized as a neuraminate 9-O-acetylesterase: (i) it inactivated the receptors for influenza C virus on chicken erythrocytes; (ii) it had acetylesterase activity as indicated by the release of acetate from bovine submandibullary mucin; (iii) monoclonal antibodies directed against gp inhibited the acetylesterase activity of influenza C virus. Although purified gp was unable to agglutinate chicken red blood cells, it blocked haemagglutination by viruses. This finding as well as the haemagglutination inhibition activity of monoclonal anti-gp antibodies indicate that gp is also responsible for the haemagglutinating activity of influenza C virus. Thus, as the influenza C glycoprotein is the only myxovirus glycoprotein with three different activities, we propose the designation HEF in order to describe its function as a haemagglutination (H), an esterase (E) and a fusion factor (F).