Properties of subviral particles of hepatitis B virus
Properties of subviral particles of hepatitis B virus
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DOI：
10.1128/jvi.00561-08
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发表时间：
2008-08-01
影响因子：
5.4
通讯作者：
Taylor, John
中科院分区：
文献类型：
--
作者：
Chai, Ning;Chang, Ho Eun;Taylor, John
在感染B型肝炎病毒（HBV）的患者血清中，除感染性颗粒外，还存在过量（通常为1，000 - 100，000倍）的空亚病毒颗粒（SVP），其仅由HBV包膜蛋白组成，呈相对较小的球体和长度可变的细丝形式。丁型肝炎病毒（HDV）组装也使用HBV的包膜蛋白来产生感染性颗粒。速率带沉降法用于研究仅产生SVT、HDV + SVP和HBV + SVP的肝细胞系释放的颗粒。在不存在HBV或HDV的情况下制备的SVP进一步通过电子显微镜检查。它们有效地结合到肝素柱上，与结合细胞表面糖胺聚糖的能力一致。然而，与作为有效抑制剂的可溶形式的HBV包膜蛋白不同，SVP不抑制HBV和HDV感染原代人肝细胞的能力。
In the sera of patients infected with hepatitis B virus (HBV), in addition to infectious particles, there is an excess (typically 1,000- to 100,000-fold) of empty subviral particles (SVP) composed solely of HBV envelope proteins in the form of relatively smaller spheres and filaments of variable length. Hepatitis delta virus (HDV) assembly also uses the envelope proteins of HBV to produce an infectious particle. Rate-zonal sedimentation was used to study the particles released from liver cell lines that produced SVT only, HDV plus SVP, and HBV plus SVP. The SVP made in the absence of HBV or HDV were further examined by electron microscopy. They bound efficiently to heparin columns, consistent with an ability to bind cell surface glycosaminoglycans. However, unlike soluble forms of HBV envelope protein that were potent inhibitors, the SVP did not inhibit the ability of HBV and HDV to infect primary human hepatocytes.