Changes in the morphology and polysaccharide content of Microcystis aeruginosa (Cyanobacteria) during flagellate grazing
Changes in the morphology and polysaccharide content of Microcystis aeruginosa (Cyanobacteria) during flagellate grazing
复制标题
鞭毛虫放牧期间铜绿微囊藻（蓝藻）形态和多糖含量的变化
DOI：
10.1111/j.1529-8817.2008.00502.x
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发表时间：
2008-06-01
影响因子：
2.9
通讯作者：
Cao, Huansheng
中科院分区：
文献类型：
--
作者：
Yang, Zhou;Kong, Fanxiang;Cao, Huansheng
研究铜绿微囊藻（Microcystis aeruginosa（Kutz.）库茨在鞭毛虫放牧期间，M.在受控实验室条件下，将铜绿假单胞菌暴露于放牧Ochromonas sp. 9 d。M.铜绿假单胞菌对鞭毛虫的取食反应活跃，形成菌落，大多数菌落由几个或几十个细胞组成，表明鞭毛虫的取食可能是铜绿假单胞菌菌落形成的生物因素之一。铜绿。菌落形成时，细胞表面超微结构发生变化，细胞壁表面多糖层变厚。这一变化表明，M.铜绿假单胞菌细胞在鞭毛虫放牧压力下增加。结果表明，在不同浓度下，M. coli菌落中可溶性胞外多糖（sEPS）、结合型胞外多糖（bEPS）和总多糖（TPS）的含量均高于对照组。铜绿假单胞菌的表达与单细胞相比显著增加。这一发现表明，细胞表面EPS数量的增加可能在保持M。铜绿假单胞菌细胞聚集在一起形成菌落。
To investigate the changes in the morphology and polysaccharide content of Microcystis aeruginosa (Kutz.) Kutz. during flagellate grazing, cultures of M. aeruginosa were exposed to grazing Ochromonas sp. for a period of 9 d under controlled laboratory conditions. M. aeruginosa responded actively to flagellate grazing and formed colonies, most of which were made up of several or dozens of cells, suggesting that flagellate grazing may be one of the biotic factors responsible for colony formation in M. aeruginosa. When colonies were formed, the cell surface ultrastructure changed, and the polysaccharide layer on the surface of the cell wall became thicker. This change indicated that synthesis and secretion of extracellular polysaccharide (EPS) of M. aeruginosa cells increased under flagellate grazing pressure. The contents of soluble extracellular polysaccharide (sEPS), bound extracellular polysaccharide (bEPS), and total polysaccharide (TPS) in colonial cells of M. aeruginosa increased significantly compared with those in single cells. This finding suggested that the increased amount of EPS on the cell surface may play a role in keeping M. aeruginosa cells together to form colonies.