Glioblastoma U-87MG tumour cells suppressed by ZnO folic acid-conjugated nanoparticles: an in vitro study
Glioblastoma U-87MG tumour cells suppressed by ZnO folic acid-conjugated nanoparticles: an in vitro study
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DOI：
10.1080/21691401.2019.1577889
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Jameie, Manasadat
中科院分区：
文献类型：
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作者：
Marfavi, Zahra Hamod;Farhadi, Mona;Jameie, Manasadat
多形性胶质母细胞瘤（GBM）是中枢神经系统最常见的恶性肿瘤。GBM的治疗包括最大限度的肿瘤切除和化疗。最近的进展强调了纳米颗粒的使用，例如氧化锌纳米颗粒（ZnO-NP）。结合有叶酸（FA）的ZnO纳米颗粒容易穿过细胞膜。本研究将ZnO纳米粒子-FA应用于GBM U87 MG细胞系。采用溶胶-凝胶法合成了ZnO NPs-FA。培养GBM U87 MG和星形胶质细胞1321 N1细胞系，并分为对照组、假手术组和ZnO NPs-FA组。MTT法检测细胞活力，ROS法和流式细胞仪检测细胞活力。纳米颗粒的尺寸是
Glioblastoma Multiform (GBM) known as the most common CNS malignant tumour. Therapy for GBM includes maximum tumour resection and chemotherapy. Recent advances have emphasized the use of nanoparticles, such as zinc oxide nanoparticles (ZnO-NPs). Conjugated ZnO NPs with folic acid (FA) easily pass through cell membrane. In the present study, ZnO NPs-FA applied to GBM U87MG cell line. ZnO NPs-FA synthesized according to the sol-gel method. The GBM U87MG and astrocytes 1321N1 cell lines cultured and divided into control, sham and ZnO NPs-FA groups. MTT assay used for the cell viability, and ROS assay and flow cytometry exploited. The size of nanoparticles was