Latitudinal Variation in the Molecular Diversity and Community Composition of Symbiodiniaceae in Coral From the South China Sea
Latitudinal Variation in the Molecular Diversity and Community Composition of Symbiodiniaceae in Coral From the South China Sea
复制标题
南海珊瑚共生科分子多样性和群落组成的纬度变化
DOI：
10.3389/fmicb.2019.01278
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发表时间：
2019-06-18
影响因子：
5.2
通讯作者：
Wang, Yinghui
中科院分区：
文献类型：
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作者：
Chen, Biao;Yu, Kefu;Wang, Yinghui
由于人类活动造成的气候变化，全世界的珊瑚礁正在持续减少。共生藻科的分子多样性和生态地理格局的研究，对于了解珊瑚-藻类共生的适应潜力和恢复力至关重要。采用下一代测序技术对中国南海（SCS）8个珊瑚栖息地的178个造礁珊瑚样本的共生藻科rDNA内转录间隔区2标记基因进行了分析。共鉴定出3个共生藻科属，Cladocopia，Durusdinium和Gerakladium，以及31个优势共生藻科类型。共生藻科的丰富度，多样性和社区组成根据纬度不同，中，低纬度珊瑚礁（IR和LR）有较高的共生藻科的丰富度和多样性比高纬度珊瑚栖息地（HC和HR）。PERMANOVA分析发现SCS中共生藻科群落组成存在显著差异（F = 14.75，R-2 = 0.20，p = 0.001 < 0.01）。HC和HR的优势共生藻科类型主要为C1，IR的优势共生藻科类型主要为Cl /Cspc/C50/C15和D1，LR的优势共生藻科类型主要为C3 u和C15。典范对应分析表明，共生藻科不同类型的相对多度受多种环境因子的影响。系统发育分析表明，共生藻科的枝藻具有共同的祖先，具有相似的环境适应性。基于这些结果，我们认为，珊瑚宿主物种发挥了相对较小的作用，在身份的优势共生藻科类型。因此，共生蕨科的地理分布格局可能主要受环境因素的影响。我们的研究提供了一个全面的概述共生藻科在南海，珊瑚群落和珊瑚礁广泛分布在不同纬度地区，并具有可变的环境条件。研究结果将为进一步研究共生藻科的区域多样性和珊瑚-共生藻科共生体的生态恢复力提供依据。
Coral reefs are continuing to decline worldwide due to anthropogenic climate change. The study of the molecular diversity and biogeographical patterns of Symbiodiniaceae, is essential to understand the adaptive potential and resilience of coral-algal symbiosis. Next generation sequencing was used to analyze the Symbiodiniaceae rDNA internal transcribed spacer 2 marker genes from 178 reef-building coral samples in eight coral habitats across approximately 13 degrees of latitude in the South China Sea (SCS). A total of three Symbiodiniaceae genera, Cladocopium, Durusdinium, and Gerakladium, as well as 31 dominant Symbiodiniaceae types, were identified. Symbiodiniaceae richness, diversity, and community composition varied according to latitude; intermediate and low latitude coral reefs (IR and LR) have higher Symbiodiniaceae richness and diversity than high latitude coral habitats (HC and HR). A PERMANOVA analysis found significant differences in the Symbiodiniaceae community composition in the SCS (F = 14.75, R-2 = 0.20, p = 0.001 < 0.01). The major dominant Symbiodiniaceae types were C1 in the HC and the HR, Cl /Cspc/C50/C15 and D1 in the IR, and C3u and C15 in the LR. Canonical correspondence analysis showed that the relative abundance of different Symbiodiniaceae types is affected by multiple environmental factors. Phylogenetic analysis indicated that the Symbiodiniaceae type Cladocopium, which shared common ancestors, shows similar environmental adaptability. Based on these results, we suggest that coral host species played a relatively small role in the identity of the dominant Symbiodiniaceae type. Therefore, the biogeographical patterns of Symbiodiniaceae may be mainly affected by environmental factors. Our research provides a comprehensive overview of the biogeography of Symbiodiniaceae in the SCS, where coral communities and reefs are widely distributed across different latitude regions and have variable environmental conditions. Our data will provide support for further study of the regional diversification of Symbiodiniaceae and the ecological resilience of the coral-Symbiodiniaceae symbioses.