KIAA0101 (OEACT-1), an expressionally down-regulated and growth-inhibitory gene in human hepatocellular carcinoma.
KIAA0101 (OEACT-1), an expressionally down-regulated and growth-inhibitory gene in human hepatocellular carcinoma.
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DOI：
10.1186/1471-2407-6-109
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发表时间：
2006-04-29
期刊：
影响因子：
3.8
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Guo M;Li J;Wan D;Gu J
我们以前的cDNA阵列结果表明，KIAA 0101作为一个差异表达的基因在人肝细胞癌（HCC）与非癌肝。然而，有必要在蛋白水平上研究其在肝癌中的表达及其对肝癌细胞生长的生物学作用。通过Western blot和组织芯片比较KIAA 0101蛋白在来自相同患者的成对人HCC和非癌肝组织中的表达水平。采用双荧光图像分析和富集线粒体蛋白的Western印迹分析对其亚细胞定位进行了研究。体外细胞生长曲线用于检查KIAA 0101在HCC细胞中过表达的效果。在KIAA 0101 cDNA转染后，使用FACS分析通过pMACSKKII系统分离的KIAA 0101表达阳性（+）和阴性（-）细胞群体中的细胞周期模式。Westernblot检测结果显示，与癌旁肝组织相比，25例HCC组织中KIAA 0101蛋白表达下调。组织阵列在161个配对样本中也表现出相同的模式。KIAA 0101主要定位于线粒体，部分定位于细胞核。KIAA 0101基因转染能抑制肝癌细胞的生长。在细胞周期分析中，它可以将细胞阻滞在G1期到S期的转变。KIAA 0101蛋白在HCC中表达下调。该基因在体外可抑制肝癌细胞的生长，可能是通过阻断细胞周期而实现的。
Our previous cDNA array results indicated KIAA0101 as one of the differentially expressed genes in human hepatocellular carcinoma (HCC) as compared with non-cancerous liver. However, it is necessary to study its expression at protein level in HCC and its biological function for HCC cell growth. Western blot and tissue array were performed to compare KIAA0101 protein expression level in paired human HCC and non-cancerous liver tissues from the same patients. Investigation of its subcellular localization was done by using dual fluorescence image examination and enriched mitochondrial protein Western blot analysis. The in vitro cell growth curve was used for examing the effect of over-expression of KIAA0101 in HCC cells. FACS was used to analyze the cell cycle pattern in KIAA0101 expression positive (+) and negative (-) cell populations isolated by the pMACSKKII system after KIAA0101 cDNA transfection. Western blot showed KIAA0101 protein expression was down-regulated in HCC tissues as compared with their counterpart non-cancerous liver tissues in 25 out of 30 cases. Tissue array also demonstrated the same pattern in 161 paired samples. KIAA0101 was predominantly localized in mitochondria and partially in nuclei. KIAA0101 cDNA transfection could inhibit the HCC cell growth in vitro. In cell cycle analysis, it could arrest cells at the G1 to S phase transition. KIAA0101 protein expression was down-regulated in HCC. This gene could inhibit the HCC cell growth in vitro and presumably by its blocking effect on cell cycle.