Macroporous and nanofibrous hyaluronic acid/collagen hybrid scaffold fabricated by concurrent electrospinning and deposition/leaching of salt particles
Macroporous and nanofibrous hyaluronic acid/collagen hybrid scaffold fabricated by concurrent electrospinning and deposition/leaching of salt particles
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DOI：
10.1016/j.actbio.2008.06.008
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发表时间：
2008-11-01
影响因子：
9.7
通讯作者：
Park, Tae Gwan
中科院分区：
文献类型：
--
作者：
Kim, Taek Gyoung;Chung, Hyun Jung;Park, Tae Gwan
采用静电纺丝结合盐浸技术制备了三维大孔纳米纤维透明质酸支架。将HA和胶原以10重量%的浓度溶解在氢氧化钠（NaOH）/N，N-二甲基甲酰胺（DMF）溶剂混合物中。并通过许多微射流演变及其随后的垂直生长的组合效应成功地电纺成具有柔软、蓬松结构的纤维网。据我们所知，形成广泛的蓬松的纤维状形态是第一次作为一个单一的路线已被用来自发地产生一个3-D纳米纤维结构。通过在静电纺丝和随后的化学交联和盐浸出期间同时沉积盐颗粒作为致孔剂，可以产生保持大孔和纳米纤维几何形状的水溶胀性HA基支架。牛软骨细胞在HA/胶原支架上培养，以评估支架的细胞相容性。结果表明，细胞粘附和增殖的胶原蛋白的含量成比例地增强，和种子的软骨细胞保持成软骨细胞形态的圆形特征。(C)2008 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
A three-dimensional (3-D) macroporous and nanofibrous hyaluronic acid (HA) scaffold was fabricated by an electrospinning process combined with a salt leaching technique. HA and collagen were dissolved in a sodium hydroxide (NaOH)/N,N-dimethyl formamide (DMF) solvent mixture at a concentration of 10 wt.% and successfully electrospun into a nanofiber web with a soft, fluffy structure by the combined effects of numerous minijet evolutions and their subsequent vertical growth. To our knowledge, the formation of an extensive fluffy nanofiber morphology is the first time as a single route has been used to spontaneously generate a 3-D nanofibrous structure. By the simultaneous deposition of salt particulates as a porogen during electrospinning and subsequent chemical cross-linking and salt leaching, a water-swellable HA-based scaffold retaining a macroporous and nanofibrous geometry could be produced. Bovine chondrocytes were cultured on the HA/collagen scaffold to assessing the scaffold's cytocompatibility. The results revealed that cellular adhesion and proliferation were enhanced in proportion to the content of collagen, and the seeded chondrocytes maintained the roundness characteristic of a chondroblastic morphology. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.