Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli.
Structure and expression of the gene locus encoding the phosphatidylglycerophosphate synthase of Escherichia coli.
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发表时间：
1986-01
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通讯作者：
Alampallam S. Gopalakrishnan;Yang-Chang ChenS;Meg Temkin;William Dowhang
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作者：
Alampallam S. Gopalakrishnan;Yang-Chang ChenS;Meg Temkin;William Dowhang
本文提出了明确的结果，建立了直接的基因-蛋白质产物之间的关系pgsA基因和磷脂酰甘油磷酸合酶的大肠杆菌。预测的蛋白质序列来自确定的DNA序列的pgsA是在密切的协议与氨基酸组成和部分确定的氨基酸序列的纯化酶。纯化的合酶具有与通过质粒定向转录-翻译系统制备的基因产物相同的表观分子量。pgsA基因的质粒携带拷贝也能够在体外表达具有酶活性的合酶。DNA序列分析已经建立了准确的线性关系之间的uvrC，pgsA，和glyW位点，并显示，这三个基因在同一方向转录。这三个基因的末端编码区也与相邻基因的转录调控元件共享共同序列。
This paper presents definitive results which establishes a direct gene-protein product relationship between the pgsA gene and the phosphatidylglycerophosphate synthase of Escherichia coli. The predicted protein sequence derived from the determined DNA sequence of pgsA is in close agreement with the amino acid composition and partially determined amino acid sequence of the purified enzyme. The purified synthase has the same apparent molecular mass as the gene product made by a plasmid-directed transcription-translation system. The plasmid-borne copy of the pgsA gene is also capable of expressing enzymatically active synthase in vitro. The DNA sequence analysis has established the exact linear relationship between the uvrC, pgsA, and glyW loci and revealed that these three genes are transcribed in the same direction. The terminal coding regions of these three genes also share common sequences with transcriptional regulatory elements for the adjacent genes.