A new organic solvent for use in the clearing of tissues. I. Soft tissue histology
A new organic solvent for use in the clearing of tissues. I. Soft tissue histology
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DOI：
10.1002/ar.1091970302
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发表时间：
1980-07
期刊：
影响因子：
--
通讯作者：
H. Wishe;M. Roy;S. Piliero
中科院分区：
文献类型：
--
作者：
H. Wishe;M. Roy;S. Piliero
组织醇是一种闪点为124°F(T.C.C.)的合成芳香烃的不可燃溶剂混合物。与通常用作清洗剂和脱烷剂的二甲苯等其他有机溶剂相比，它具有较低的蒸气压和蒸发速率。虽然二甲苯和组织醇在制备永久固定的染色切片时都能有效地清除和脱脂软组织，但在许多情况下，组织醇似乎是选择的溶剂：组织更容易切片；细胞边界和细胞表面修饰最明显；细胞质嗜酸性粒细胞更明显；核细节得到改善。最重要的是，在组织学和病理学实验室中使用组织醇是一种更安全和更有效的溶剂。
Histosol is a non‐flammable solvent mixture of synthetic aromatic hydrocarbons with a flash point of 124°F (T.C.C.). It has a lower vapor pressure and evaporation rate than other organic solvents, such as xylene, routinely used as clearing and deparaffinizing agents. Although both xylene and Histosol clear and deparaffinize soft organ tissues effectively in the preparation of permanently mounted stained slides, Histosol appears, in many instances, to be the choice solvent: tissues are easier to section; cell bordrs and cell surface modifications are most distinct; cytoplasmic eosinophilia is more vivid; and nuclear detail is improved. Of prime importance, Histosol is a safer and more efficient solvent for use in histological and pathological laboratories.