Biological nitrification inhibition by rice root exudates and its relationship with nitrogen-use efficiency.
Biological nitrification inhibition by rice root exudates and its relationship with nitrogen-use efficiency.
复制标题
DOI：
10.1111/nph.14057
复制
发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Li Sun;Yufang Lu;Fangwei Yu;H. Kronzucker;W. Shi
中科院分区：
文献类型：
--
作者：
Li Sun;Yufang Lu;Fangwei Yu;H. Kronzucker;W. Shi
土壤中的微生物硝化作用是农业系统氮素损失的主要原因。一些植物可以分泌作为生物硝化抑制剂(BNI)的有机物质，已经鉴定和表征了少量的BNI。然而，几乎没有研究集中在重要的粮食作物--水稻上。在这里，研究了19个水稻品种对关键硝化细菌欧洲亚硝化单胞菌的BNI潜力。研究发现，两种水稻品种的分泌物都具有很强的BNI潜力。中九25(ZJ25)和武云粳7号(WYJ7)是水稻品种中最有效的两个品种。鉴定了一种新的硝化抑制剂1，9-正十二醇，它能阻断氨氧化的氨单加氧酶途径，其80%的有效剂量(ED80)为90 ngμL-1。植物氮素利用效率(NUE)采用15N标记法测定。相关分析表明，根分泌物的BNI能力和1，9-癸二醇量均与植物对铵的利用效率和对铵的偏好呈正相关。这些发现为了解土壤氮素循环中的植物-细菌相互作用提供了重要的新见解，并提高了我们在氮素利用背景下对水稻BNI能力的理解。
Microbial nitrification in soils is a major contributor to nitrogen (N) loss in agricultural systems. Some plants can secrete organic substances that act as biological nitrification inhibitors (BNIs), and a small number of BNIs have been identified and characterized. However, virtually no research has focused on the important food crop, rice (Oryza sativa). Here, 19 rice varieties were explored for BNI potential on the key nitrifying bacterium Nitrosomonas europaea. Exudates from both indica and japonica genotypes were found to possess strong BNI potential. Older seedlings had higher BNI abilities than younger ones; Zhongjiu25 (ZJ25) and Wuyunjing7 (WYJ7) were the most effective genotypes among indica and japonica varieties, respectively. A new nitrification inhibitor, 1,9-decanediol, was identified, shown to block the ammonia monooxygenase (AMO) pathway of ammonia oxidation and to possess an 80% effective dose (ED80 ) of 90 ng μl-1 . Plant N-use efficiency (NUE) was determined using a 15 N-labeling method. Correlation analyses indicated that both BNI abilities and 1,9-decanediol amounts of root exudates were positively correlated with plant ammonium-use efficiency and ammonium preference. These findings provide important new insights into the plant-bacterial interactions involved in the soil N cycle, and improve our understanding of the BNI capacity of rice in the context of NUE.