A non-invasive method to isolate the neuronal linage from the nasal epithelium from schizophrenic and bipolar diseases
A non-invasive method to isolate the neuronal linage from the nasal epithelium from schizophrenic and bipolar diseases
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DOI：
10.1016/j.jneumeth.2011.07.009
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发表时间：
2011-09-30
影响因子：
3
通讯作者：
Dominguez-Alonso, A.
中科院分区：
文献类型：
--
作者：
Benitez-King, G.;Riquelme, A.;Dominguez-Alonso, A.
脑成像和组织病理学研究表明，神经发育异常在精神分裂症（SZ）和双相情感障碍（BD）的病因中起关键作用。人类嗅上皮在整个生命过程中都有新的神经元形成和成熟。因此，嗅上皮被提议作为一种研究神经发育变化的模型，特别是在一些精神疾病中。然而，以前的研究是使用从精神分裂症和双相情感障碍患者死后或在麻醉下获取的嗅上皮活检进行的。在这项工作中，我们开发了一种新方法，通过从健康对照者和门诊患者鼻腔的中鼻甲外侧前部区域脱落来获取有活力的神经前体细胞。细胞被增殖以建立神经前体库。解冻的细胞显示出发育中神经元典型的细胞骨架表型。它们在2 mM二丁酰 - 环磷酸腺苷存在的情况下也保留了分化能力，并在培养中维持了电压门控Ca²⁺电流。此外，从精神分裂症和双相情感障碍患者获得的培养脱落细胞中，神经元成熟阶段的比例得以维持。数据支持从鼻腔脱落细胞中获得的神经前体细胞是一个极好的实验模型，可用于后续对精神分裂症和双相情感障碍的生物标志物、神经发育和病理生理学中细胞变化的研究。（C）2011爱思唯尔公司。保留所有权利。
Brain imaging and histopathological studies suggest that neurodevelopmental anomalies play a key role in the etiology of schizophrenia (SZ) and bipolar disorder (BD). New neuron formation and maturation occur in human olfactory epithelium throughout life. Therefore, the olfactory epithelium has been proposed as a model to study alterations in neurodevelopment, particularly in some psychiatric diseases. However, former studies were done with olfactory epithelium biopsies taken post mortem or under anesthesia from patients with SZ and BD. In this work we have developed a new method to obtain viable neural precursors by exfoliation of the anterior region of the medial lateral turbinate of the nasal cavity from healthy controls, and ambulatory patients. Cells were propagated to establish neural precursor banks. Thawed cells showed cytoskeletal phenotypes typical of developing neurons. They also conserved the ability to differentiate in presence of 2 mM dibutyril-cyclic adenosine monophosphate, and maintained voltage-operated Ca2+ currents in culture. Moreover, proportions of neuronal maturation stages were maintained in cultured exfoliates obtained from SZ and BD patients. Data support that neural precursors obtained from a nasal exfoliate are an excellent experimental model to later approach studies on biomarkers, neural development and cellular alterations in the pathophysiology of SZ and BD. (C) 2011 Elsevier B.V. All rights reserved.