Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only
Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only
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DOI：
10.1016/s0168-1656(00)00287-x
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发表时间：
2000-08-25
影响因子：
4.1
通讯作者：
Jacquin-Dubreuil, A
中科院分区：
文献类型：
--
作者：
Bonhomme, V;Laurain-Mattar, D;Jacquin-Dubreuil, A
颠茄叶盘感染的野生菌株发根农杆菌15834窝藏的Ri-TL-DNA和解除武装的根癌农杆菌菌株窝藏只有vol ABC和npt II基因的建设。建立了13条根系，并对其生长速率和生物碱生产率进行了研究，以评估vol基因在形态分化和托品烷生物碱形成中的可能作用。这13条根系的生长速度差异很大。根系生物量增加了75倍。用A. rhizogenes 15834和A.根癌农杆菌最后一个积累了4（1.1 mg g（-1）DW）和27（8 mg g（-1）DW）倍以上的生物碱比完整的根（0.3 mg g（-1）DW）。这项工作表明vol ABC基因足以增加A.颠茄毛状根培养物。(C)2000 Elsevier Science B. V.保留所有权利。
Atropa belladonna leaf disks were infected by a wild strain Agrobacterium rhizogenes 15834 harboring the Ri-TL-DNA and by a disarmed Agrobacterium tumefaciens strain harboring a construction with only vol ABC and npt II genes. Thirteen root lines were established and examined for their growth rate and alkaloid productivity to evaluate the possible role of vol genes in morphological differentiation and in tropane alkaloid formation. A great diversity has been observed in the growth rate of these 13 root lines. The root biomass increased up to 75 times. The total alkaloid contents were similar in the root lines obtained by infection with A. rhizogenes 15834 and A. tumefaciens rol ABC. The last ones accumulated between 4 (1.1 mg g(-1) DW) and 27 (8 mg g(-1) DW) times more alkaloids than the intact roots (0.3 mg g(-1) DW). This work has shown that the vol ABC genes were sufficient to increase tropane alkaloid production in A. belladonna hairy root cultures. (C) 2000 Elsevier Science B.V. All rights reserved.