Evaluation of a monoclonal antibody-based approach for the selection of foot-and-mouth disease (FMD) vaccine strains
Evaluation of a monoclonal antibody-based approach for the selection of foot-and-mouth disease (FMD) vaccine strains
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DOI：
10.1016/j.vetmic.2007.06.022
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发表时间：
2008-01-01
影响因子：
3.3
通讯作者：
Paton, D. J.
中科院分区：
文献类型：
--
作者：
Mahapatra, M.;Aggarwal, N.;Paton, D. J.
口蹄疫(FMD)病毒以七种血清型存在，其中有许多变异，需要仔细选择疫苗毒株。目前，基于多克隆疫苗抗血清的血清学检测系统被广泛用于这一选择。然而，所使用的匹配抗血清的固有变异性使得测试的重复性很差，难以解释。在这项研究中，我们探索了用基于单抗的方法取代或补充基于多克隆抗体(Pab)的方法的可能性。对针对两个O型疫苗毒株的单抗组合进行了检测，以检测它们与从1991年至2001年的10年间分离的22种野毒的反应性。发现抗原位点2包含一个以上的表位。分析了含有第2抗原点的衣壳蛋白VP2的序列变异，发现第79和134位氨基酸残基对第2位单抗的结合有很大影响。基于单抗反应性的抗原匹配预测与基于多抗的“金标准”方法的结果并不密切相关，结论是需要更广泛的单抗小组来识别存在于口蹄疫病毒表面的所有保护性表位，并更好地了解那些在赋予保护中重要的表位。(C)2007年，爱思唯尔出版。
Foot-and-mouth disease (FMD) virus exists as seven serotypes within which are numerous variants necessitating careful selection of vaccine strains. Currently, a serological assay system based on the use of polyclonal vaccine antisera is widely used for this selection. However, inherent variability in the matching antisera used makes the tests poorly reproducible and difficult to interpret. In this study, we have explored the possibility of replacing or supplementing the polyclonal antibody (PAb)-based method with one based on use of monoclonal antibodies (MAb). Panels of MAbs raised against two serotype O vaccine strains were examined for reactivity with 22 field viruses, isolated over a 10-year period between 1991 and 2001. Antigenic site 2 was found to comprise more than one epitope. The sequence variation in capsid protein VP2 harbouring antigenic site 2 was analysed and the amino acid residues at positions 79 and 134 appeared to greatly influence the binding of site 2 MAbs. Prediction of antigenic match based on MAb reactivity did not correlate closely with the results of a PAb-based "gold-standard" method and it was concluded that a wider panel of MAbs are needed that recognise all protective epitopes present on the surface of FMD virus together with a better understanding of those epitopes which are important in conferring protection. (C) 2007 Published by Elsevier B.V.