Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D. Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D. Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry
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通过高效液相色谱结合离子阱飞行时间质谱法鉴定从 Halenia elliptica D. Don 中分离的生物活性呫吨酮的代谢物
DOI：
10.1016/j.cclet.2010.12.021
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发表时间：
2011-07
影响因子：
9.1
通讯作者：
Liu, Xiao Wei
中科院分区：
文献类型：
--
作者：
Yeung, John H. K.;Che, Chun Tao;Wang, Yan;Shi, Jian Gong;Feng, Ru;Liu, Xiao Wei
对从藏药椭圆花锚中分离得到的两种生物活性成分1-羟基-2，3，5-三甲氧基口山酮（HM-1）和1-羟基-2，3，4，7-四甲氧基口山酮（HM-2）进行了代谢研究。唐，在体外大鼠肝微粒体中。高效液相色谱-离子阱飞行时间质谱联用技术应用LCMSn-ESI-IT-TOF对HM-1和HM-2的代谢产物进行在线分析，共鉴定出5种代谢产物，分别为1，5-二羟基-2，3-二甲氧基口山酮（HM-5），1，7-二羟基-2，3，4-三甲氧基口山酮（HM-9），1，4，7-三羟基-2，3-二甲氧基口山酮（HM-10），1，4-二羟基-2，3，7-三甲氧基氧杂蒽酮（HM-11）和1，2-二羟基-3，4，7-三甲氧基氧杂蒽酮（HM-12）。在这些代谢产物中，HM-9、HM-11和HM-12互为异构体。结果表明，HM-1和HM-2在体外大鼠微粒体中发生了去甲基化的I相代谢反应。
Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone (HM-1) and 1-hydroxy-2,3,4,7-tetramethoxyxanthone (HM-2), which are two biologically active ingredients isolated from the Tibetan herb,Halenia ellipticaD. Don., in rat liver microsomesin vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMSn-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone (HM-5), 1,7-dihydroxy-2,3,4-trimethoxyxanthone (HM-9), 1,4, 7-trihydroxy-2,3-dimethoxyxanthone (HM-10), 1,4-dihydroxy-2,3,7-trimethoxyxanthone (HM-11) and 1,2-dihydroxy-3,4,7-trimethoxyxanthone (HM-12). Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomesin vitro.
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影响因子：
6.1
作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung
通讯作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung
影响因子：
3.8
作者：
D. L. Dreyer;J. Bourell
通讯作者：
D. L. Dreyer;J. Bourell
DOI：
10.1016/j.phymed.2009.03.015
发表时间：
2009-12
期刊：
Phytomedicine : international journal of phytotherapy and phytopharmacology
影响因子：
--
作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung
通讯作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung
影响因子：
6.1
作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung
通讯作者：
Yan Wang;Jiangong Shi;Mu-zou Wang;C. Che;J. Yeung