Characterization of luciferases and its paralogue in the Panamanian luminous click beetle Pyrophorus angustus: A click beetle luciferase lacks the fatty acyl-CoA synthetic activity
Characterization of luciferases and its paralogue in the Panamanian luminous click beetle Pyrophorus angustus: A click beetle luciferase lacks the fatty acyl-CoA synthetic activity
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DOI：
10.1016/j.gene.2009.12.001
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发表时间：
2010-02-15
期刊：
影响因子：
3.5
通讯作者：
Inouye, Satoshi
中科院分区：
文献类型：
--
作者：
Oba, Yuichi;Kumazaki, Mizuho;Inouye, Satoshi
从巴拿马夜光叩甲Pyrophorus angustus（叩甲科，叩甲亚科）中分离到两个荧光素酶基因（dPaLuc和vPaLuc）和一个荧光素酶基因片段（PaLL）。dPaLuc和vPaLuc的转录本主要分布在背侧和腹侧两个部位，而PaLL的转录本则分布在背侧和腹侧两个部位。dPaLuc和vPaLuc的基因产物具有与萤火虫荧光蛋白的发光活性（λ（max）分别为536和566 nm），但没有显示出显著的脂肪酰辅酶A合成活性。另一方面，PaLL的基因产物具有脂肪酰辅酶A合成活性，但发光活性很弱。本研究发现，萤火虫荧光素酶具有发光活性和脂肪酰辅酶A合成活性。这些结果表明，祖先的脂肪酰辅酶A合成酶在Pyrophorinae谱系经历了基因复制事件，随后一个拷贝的荧光素酶的专业化。随后，荧光素酶再次复制，并且两个拷贝在其发光颜色和表达模式上发生分歧。(C)2009 Elsevier B.V.保留所有权利。
Two luciferase genes (dPaLuc and vPaLuc) and one paralogue of luciferase (PaLL) were isolated from the Panamanian luminous click beetle, Pyrophorus angustus (Elateridae, Pyrophorinae). The transcripts of dPaLuc and vPaLuc were predominantly detected in the body parts with dorsal photophore and ventral photophore, respectively, and the transcript of PaLL was detected in both parts. The gene products of dPaLuc and vPaLuc possessed luminescence activity with firefly luciferin (lambda(max) = 536 and 566 nm, respectively) but did not show significant activity of fatty acyl-CoA synthesis. On the other hand, the gene product of PaLL had fatty acyl-CoA synthetic activity with very weak luminescence activity. The catalytic properties of click beetle luciferase are different from our previous results that firefly luciferase has both luminescence activity and fatty acyl-CoA synthetic activity. These results suggested that the ancestral fatty acyl-CoA synthetase in the Pyrophorinae lineage has undergone gene duplication event, followed by specialization of one copy in luciferase. Subsequently, the luciferase was duplicated again and the two copies diverged in their luminescent color and expression pattern. (C) 2009 Elsevier B.V. All rights reserved.