Exploration of an imide capture/N,N-acyl shift sequence for asparagine native peptide bond formation
Exploration of an imide capture/N,N-acyl shift sequence for asparagine native peptide bond formation
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天冬酰胺天然肽键形成的酰亚胺捕获/N,N-酰基移位序列的探索
DOI：
10.1016/j.bmc.2013.02.053
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
O.
中科院分区：
文献类型：
--
作者：
Mhidia;R.; Boll;E.; Fécourt;F.; Ermolenko;M.; Ollivier;N.; Sasaki;K.; Crich;D.; Delpech;B.; Melnyk;O.
C-末端肽基叠氮化物与N-末端受保护的N-乙酰基肽的侧链硫代酸衍生物的酰亚胺捕获导致酰亚胺键的形成，使两个肽末端紧密接近。Nα保护基的暴露和分子内酰基迁移导致形成与天冬酰胺的天然肽键。
Imide capture of a C-terminal peptidylazide with a side-chain thioacid derivative of an N-terminally protected aspartyl peptide leads to the formation of an imide bond bringing the two peptide ends into close proximity. Unmasking of the Nαprotecting group and intramolecular acyl migration results in the formation of a native peptide bond to asparagine.