Micro-patterned nanowire surfaces encourage directional neural progenitor cell adhesion and proliferation.
Micro-patterned nanowire surfaces encourage directional neural progenitor cell adhesion and proliferation.
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DOI：
10.1166/jbn.2013.1667
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发表时间：
2013-10
影响因子：
2.9
通讯作者：
Samuel L. Bechara;K. Popat
中科院分区：
文献类型：
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作者：
Samuel L. Bechara;K. Popat
周围神经损伤在美国很常见，估计每年有20万患者接受手术治疗。自体移植是目前的黄金标准治疗方法，它要求患者通过移植身体其他部位的神经来交换功能。因此，需要研究能够再生神经组织并指导增殖的组织工程支架。在这项工作中，我们研究了微图案纳米线表面对神经元祖细胞粘附和增殖的影响。我们创建了一个简单的微图纹工艺来创建一个微图纹支架，该支架的区域缺乏宽度约为60微米的纳米形貌，边界区域具有宽度约为400微米的纳米线形貌。我们假设神经祖细胞会优先粘附在含有纳米形貌的区域，而反过来会避开缺乏纳米形貌的区域。我们的结果表明，我们可以利用这种技术来指导神经祖细胞的增殖。结果表明，培养2天后，接触边界的细胞形态变长。培养5天后，细胞聚集体的尺寸量化显示，与对照相比，它们生长的长度是微图案纳米线上的两倍。这些研究表明，神经祖细胞的生长可以通过简单地创建一个具有纳米形貌的不同线性区域的支架来指导，这些纳米形貌由光滑、坚硬、疏水的表面所包围。
Peripheral nerve damage is common in the United States, with an estimated 200,000 patients treated surgically each year. Autografts, the current gold standard treatment, require that the patient trade functionality by transplanting a nerve from elsewhere in the body. For this reason, tissue-engineering scaffolds that can regenerate nervous tissue and that can direct proliferation need to be investigated. In this work we investigated the influence of micro-patterned nanowire surfaces on neuronal progenitor cell adhesion and proliferation. We created a simple micro-patterning process to create a micro-patterned scaffold with regions lacking a nano-topography approximately 60 microns in width bordered by regions with a nanowire topography approximately 400 microns in width. We hypothesized that neural progenitor cells would preferentially adhere to the regions containing the nano-topography and in turn would avoid regions lacking the nano-topography. Our results indicate that we can direct neural progenitor cell proliferation using this technique. Furthermore, the results show that after 2 days of culture, cells contacting the boundary have elongated morphology. After 5 days of culture, quantifying dimensions on the cell aggregates reveals that they grow twice as long as they do wide on micro-patterned nanowires when compared to controls. These studies suggest that neural progenitor cell growth can be directed by simply creating a scaffold with distinct linear regions of nano-topography bordered by a smooth, hard, hydrophobic surface.