Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia
Region-specific proliferative response of neural progenitors to exogenous stimulation by growth factors following ischemia
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DOI：
10.1097/wnr.0b013e3282ff8641
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发表时间：
2008-05-28
期刊：
影响因子：
1.7
通讯作者：
Kawahara, Nobutaka
中科院分区：
文献类型：
--
作者：
Oya, Soichi;Yoshikawa, Gakushi;Kawahara, Nobutaka
在缺血后增加神经前体细胞增殖的最有效方法仍然是未知的。我们管理各种代理到大鼠脑室短暂性全脑缺血后，比较了前脑室下区（aSVZ），齿状回颗粒下区，后脑室周，和下丘脑的神经祖细胞的反应。我们证明了表皮生长因子（EGF）和成纤维细胞生长因子-2（FGF-2）的鸡尾酒管理显着增加了所有四个区域的神经祖细胞的数量。向混合物中添加Notch配体DLL 4在aSVZ和下丘脑中引起最大的祖细胞应答。我们的研究结果表明，EGF和FGF-2，与DLL 4相结合，代表了缺血后神经祖细胞库的扩展的普遍适用的方案。
The most effective way to augment neural progenitor proliferation after ischemia is still unknown. We administered various agents into the rat cerebral ventricle after transient global ischemia and compared the neural progenitor response in the anterior subventricular zone (aSVZ), dentate gyrus subgranular zone, posterior periventricle, and hypothalamus. We demonstrated that cocktail administration of epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2) remarkably increased the numbers of neural progenitors in all four regions examined. The addition of Notch ligand DLL4 to the cocktail elicited the largest progenitor response in the aSVZ and hypothalamus. Our results suggest that EGF and FGF-2, combined with DLL4, represent the universally applicable regimen for the expansion of the neural progenitor pool following ischemia.