Liquid chromatographic assay for riluzole in mouse plasma and central nervous system tissues
Liquid chromatographic assay for riluzole in mouse plasma and central nervous system tissues
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DOI：
10.1016/j.jchromb.2004.01.004
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发表时间：
2004-04-25
影响因子：
3
通讯作者：
Caccia, S
中科院分区：
文献类型：
--
作者：
Colovic, M;Zennaro, E;Caccia, S
建立了等度反相高效液相色谱法测定小鼠血浆、脑和脊髓中神经保护剂利鲁唑的浓度。该方法基于使用Bakerbond spe（TM）C8柱从血浆和中枢神经系统组织中分离化合物和内标物，具有令人满意的回收率和专属性。分离在C18柱上进行，与263 μ的UV检测器偶联。该测定在宽范围内呈线性，使用0.1 ml血浆和约100 mg脑组织的定量下限为100 ng ml（-1）或g（-1）。精密度和准确度均在HPLC含量测定的可接受限度内。该方法目前用于支持利鲁唑与其他潜在神经保护剂联合给药时在常见肌萎缩侧索硬化症动物模型（SOD 1-G93 A转基因小鼠）中的活性药理学研究。(C)2004 Elsevier B. V.保留所有权利。
An isocratic, reversed-phase high-performance liquid chromatographic procedure (HPLC) was developed for determination of the neuroprotective agent riluzole in mice plasma, brain and spinal cord. The procedure is based on isolation of the compound and the internal standard from plasma and central nervous system tissues using a Bakerbond spe(TM) C8 cartridge, with satisfactory recovery and specificity. Separation was on a C 18 column, coupled with an UV detector at 263 mu. The assay was linear over a wide range, with a lower limit of quantification of 100 ng ml(-1) or g(-1) using 0.1 ml of plasma and about 100 mg of brain tissue. The precision and accuracy were within the acceptable limits for an HPLC assay. The method is currently used to support pharmacological studies of the activity of riluzole when given in combination with other potential neuroprotective agents in an animal model of familiar amyotrophic lateral sclerosis (SOD1-G93A transgenic mice). (C) 2004 Elsevier B.V. All rights reserved.