Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel
Linker Nups connect the nuclear pore complex inner ring with the outer ring and transport channel
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DOI：
10.1038/nsmb.3084
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发表时间：
2015-10-01
影响因子：
16.8
通讯作者：
Hurt, Ed
中科院分区：
文献类型：
--
作者：
Fischer, Jessica;Teimer, Roman;Hurt, Ed
核孔复合物（NPC）介导细胞核和细胞质之间的运输。NPC由大约30个核孔蛋白（Nups）组成，其中大部分以稳定的亚复合物形式存在。这些模块如何在大型NPC框架内互连尚不清楚。在这里，我们展示了NPC模块之间如何形成超复合体的机制。Nup192内孔环复合物充当种子，Nup82外环复合物和Nsp 1通道复合物被拴系到该种子。这些亚复合物之间的连接由接头Nups内的短序列产生。保守的Nup145N是NPC模块中最通用的连接子，因为它具有Nup192、Nup170和Nup82的三个离散结合位点。我们在体外组装了大部分嗜热毛壳菌NPC原体，为整个NPC的重建迈出了一步。
Nuclear pore complexes (NPCs) mediate transport between the nucleus and cytoplasm. NPCs are composed of similar to 30 nucleoporins (Nups), most of which are organized in stable subcomplexes. How these modules are interconnected within the large NPC framework has been unknown. Here we show a mechanism of how supercomplexes can form between NPC modules. The Nup192 inner-pore-ring complex serves as a seed to which the Nup82 outer-ring complex and Nsp1 channel complex are tethered. The linkage between these subcomplexes is generated by short sequences within linker Nups. The conserved Nup145N is the most versatile connector of NPC modules because it has three discrete binding sites for Nup192, Nup170 and Nup82. We assembled a large part of a Chaetomium thermophilum NPC protomer in vitro, providing a step forward toward the reconstitution of the entire NPC.