Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression
Cinnamic acid 4-hydroxylase from Lithospermum erythrorhizon: cDNA cloning and gene expression
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DOI：
10.1007/s002990100373
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发表时间：
2001-09
影响因子：
6.2
通讯作者：
Y. Yamamura;Y. Ogihara;H. Mizukami
中科院分区：
文献类型：
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作者：
Y. Yamamura;Y. Ogihara;H. Mizukami
通过对紫草素产生菌紫草愈伤组织构建的cDNA文库的筛选，获得了两个编码植物细胞色素P450的近全长cDNA克隆(LEC4H-1和LEC4H-2)。LEC4H-1和LEC4H-2推导的氨基酸序列有91%的同源性，并与高等植物中的各种肉桂酸4-羟基酶(C4H)序列高度同源。酵母表达研究证实，P450克隆编码C4H。培养细胞中C4H转录物的水平不受环境因素的影响，包括光照、生长素的供应和茉莉酸甲酯的添加，表明这些基因的转录是结构性的，不参与这些因素对紫草素和迷迭香酸生物合成的调节。
Two near full-length cDNA clones (LEC4H-1 and LEC4H-2) encoding plant cytochrome P450 were isolated by screening a cDNA library constructed from shikonin-producingLithospermum erythrorhizoncallus cultures. The deduced amino acid sequences of LEC4H-1 and LEC4H-2 exhibited 91% identity and were highly homologous to various cinnamic acid 4-hydroxylase (C4H) sequences from higher plants. Expression studies in yeast confirmed that the P450 clones encode C4H. The level of C4H transcripts in the cultured cells was unaffected by environmental factors, including light irradiation, supply of auxin and methyl jasmonate addition, indicating that transcription of these genes is constitutive and not involved in the regulation of shikonin and rosmarinic acid biosynthesis by these factors.