Prenatal high-fat diet alters placental morphology, nutrient transporter expression, and mtorc1 signaling in rat
Prenatal high-fat diet alters placental morphology, nutrient transporter expression, and mtorc1 signaling in rat
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DOI：
10.1002/oby.21821
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发表时间：
2017-05-01
期刊：
影响因子：
6.9
通讯作者：
Tamashiro, Kellie L. K.
中科院分区：
文献类型：
--
作者：
Song, Lin;Sun, Bo;Tamashiro, Kellie L. K.
本研究的目的是确定如何大鼠胎盘和胎儿在妊娠期间对母体高脂（HF）饮食的反应，并确定可能的mechanism. MethodsPregnancy Sprague-Dawley大鼠在妊娠期间喂养标准饲料（13.5%脂肪）或HF（60%脂肪）饮食。结果HF母鼠妊娠期脂肪量增加，血浆瘦素水平升高，脂联素水平降低，糖耐量异常。HF母鼠的雄性和雌性胎仔的胎盘迷路厚度均降低。在HF男性胎盘中，葡萄糖转运蛋白3基因表达、系统A氨基酸转运蛋白（SNAT）2基因表达和SNAT 2蛋白表达通过mTORC 1 4 EBP 1分支的激活而增加。在HF女性胎盘中，胰岛素样生长因子2（IGF 2）和IGF 2受体的基因表达与喂食标准食物的女性胎盘相比升高。虽然男性和女性的胎盘产前HF饮食暴露的反应不同，男性和女性胎儿的体重没有改变母亲HF diet.ConclusionsPlacenta响应和适应母亲的代谢变化，改变胎盘层厚度，mTORC 1信号，营养转运蛋白的表达，和生长因子的性别特异性的方式。
ObjectiveThis study aimed to determine how the rat placenta and fetus respond to maternal high-fat (HF) diet during gestation and to identify the possible mechanisms.MethodsPregnant Sprague-Dawley rats were fed with standard chow (13.5% fat) or HF (60% fat) diet during gestation. Placentas were collected on gestation day 21.ResultsHF dams had greater fat mass, higher plasma leptin, lower plasma adiponectin, and impaired glucose tolerance during pregnancy. The placental labyrinth thickness was reduced in both male and female fetuses of HF dams. In HF male placentas, glucose transporter 3 gene expression, system A amino acid transporter (SNAT) 2 gene expression, and SNAT2 protein expression were increased through the activation of the mTORC1 4EBP1 branch. In HF female placentas, gene expression of insulin-like growth factor 2 (IGF2) and IGF2 receptor was elevated compared to placentas of females fed standard chow. Although male and female placentas responded differently to prenatal HF diet exposure, both male and female fetal weight was not altered by maternal HF diet.ConclusionsPlacenta responds and adapts to maternal metabolic changes by altering placental layer thickness, mTORC1 signaling, expression of nutrient transporters, and growth factors in a sex-specific manner.