Upstream sequences confer distinctive transcriptional properties on genes encoding silkgland-specific tRNAAla.
Upstream sequences confer distinctive transcriptional properties on genes encoding silkgland-specific tRNAAla.
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上游序列赋予编码丝腺特异性 tRNAAla 的基因独特的转录特性。
DOI：
10.1073/pnas.83.2.374
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发表时间：
1986
影响因子：
11.1
通讯作者：
Sprague,KU
中科院分区：
文献类型：
--
作者：
Young,LS;Takahashi,N;Sprague,KU
为了了解家蚕组织中丙氨酸tRNAs特异性积累的分子基础，我们比较了编码构成型和丝腺特异型丙氨酸tRNA的基因的转录特性。当用作同源体外转录的模板时，每类丙氨酸tRNA的基因表现非常不同。由于这些特性可能与体内相应基因的活性有关，我们希望确定负责它们的顺式作用元件。因此，我们构建了杂交的丝腺特异/构成基因，并分析了它们在体外指导转录的能力。我们发现了一个简单的模式：这两种tRNAAla基因的不同转录特性是其转录起始点上游不同正信号的结果。
To understand the molecular basis for the tissue-specific accumulation of alanine tRNAs in silkworms, we have compared the transcriptional properties of genes that encode constitutive and silkgland-specific type alanine tRNA. Genes for each class of alanine tRNA behave very differently when used as templates for homologous in vitro transcription. Since these properties are likely related to the activities of the corresponding genes in vivo, we wished to identify the cis-acting elements responsible for them. We have therefore constructed hybrid silkgland-specific/constitutive genes and have analyzed their capacity to direct transcription in vitro. We find a simple pattern: the distinctive transcriptional properties of the two kinds of tRNAAla genes are the result of different positive signals upstream from their sites of transcription initiation.