In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids.
In vitro cytotoxic drug sensitivity testing of human tumour xenografts grown as multicellular tumour spheroids.
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DOI：
10.1038/bjc.1982.296
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发表时间：
1982-12
影响因子：
8.8
通讯作者：
Peckham, M J
中科院分区：
文献类型：
--
作者：
Jones, A C;Stratford, I J;Wilson, P A;Peckham, M J
来自7名卵巢癌患者和22种不同的人类肿瘤异种移植物的肿瘤细胞代表了广泛的组织学亚型，研究了多细胞球体形成能力。球体形成仅限于来自异种移植物的细胞。在测试的22个品系中，有5个品系形成了能够独立生长的球体。组织学类型与球体形成能力之间没有明确的关系。从球体获得的肿瘤细胞的电镀效率总是大于从分离的肿瘤来源获得的细胞，在某些情况下高达6倍。有4株细胞系呈指数型生长。采用体积生长延迟法考察美伐兰、阿霉素、长春花生物碱、CCNU和顺铂的活性。不同系间药物反应的差异广泛反映了患者和体内异种移植物的反应。
Tumour cells from 7 patients with ovarian carcinoma and from 22 different human tumour xenografts representing a wide range of histological sub-types have been examined for multicellular spheroid forming ability. Spheroid formation was limited to cells derived from xenografts. Of the 22 lines tested, 5 formed spheroids capable of growth in isolation. There was no clear relationship between histological type and spheroid-forming ability. The plating efficiency of tumour cells obtained from spheroids was always greater than for the cells obtained from the dissociated tumour of origin and was in some cases as much as 6-fold greater. Spheroid growth was nearly exponential for 4 cell lines. Volume growth delay was used to investigate the activity of melphalan, adriamycin, the Vinca alkaloids, CCNU and cisplatin. Differences between lines in drug response broadly reflected patient and in vivo xenograft response.