Culturing the marine cyanobacterium Prochlorococcus
Culturing the marine cyanobacterium Prochlorococcus
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DOI：
10.4319/lom.2007.5.353
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发表时间：
2007-10-01
影响因子：
2.7
通讯作者：
Chisholm, Sallie W.
中科院分区：
文献类型：
--
作者：
Moore, Lisa R.;Coe, Allison;Chisholm, Sallie W.
原氯球菌是公海生态系统中数量上占主导地位的初级生产者。对培养的分离物和海洋样本中的原氯球菌基因组序列的分析已经扩大了人们对这种微小细胞的研究兴趣，并正在努力将其发展成为研究海洋微生物生态的模式系统。这些努力的一个关键组成部分是开发系统的培养方法，这将促进原氯球菌分布到可能有兴趣研究它的不同实验室。本文提供了维持原氯球菌培养的详细方法，包括比较了两种人工海水培养基和一种使用天然海水基质的标准培养基上细胞的生长速度。还描述了琼脂平板培养、超低温保存、获得新的分离物的程序，以及与培养体积和碳限制相关的问题。
Prochlorococcus is the numerically dominant primary producer in open ocean ecosystems. Analysis of Prochlorococcus genome sequences from cultured isolates and ocean samples has broadened interest in studying this tiny cell, and efforts are underway to develop it into a model system for studying marine microbial ecology. A critical component of these efforts has been the development of systematic culturing methods that will facilitate the distribution of Prochlorococcus to diverse labs that may be interested in studying it. This paper provides detailed methods for maintaining cultures of Prochlorococcus, including a comparison of growth rates of cells on two artificial seawater media and on a standard medium that uses a natural seawater base. Procedures for agar plating, cryopreservation, obtaining new isolates, and issues associated with culture volume and carbon limitation also are described.