Molecular mechanisms of an antimicrobial peptide piscidin (Lc-pis) in a parasitic protozoan, Cryptocaryon irritans
Molecular mechanisms of an antimicrobial peptide piscidin (Lc-pis) in a parasitic protozoan, Cryptocaryon irritans
复制标题
寄生原生动物刺激白点虫中抗菌肽 piscidin (Lc-pis) 的分子机制
DOI：
10.1186/s12864-018-4565-5
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发表时间：
2018-03-12
期刊：
影响因子：
4.4
通讯作者：
Zheng, Weiqiang
中科院分区：
文献类型：
--
作者：
Chen, Ruanni;Mao, Yong;Zheng, Weiqiang
背景：刺激隐核虫是一种专性寄生性纤毛虫原虫，可感染多种重要的海水养殖鱼类，造成很高的致死率和经济损失。目前用化学品或抗生素控制这种寄生虫的方法被广泛认为是对环境有害的。具有广谱抗细菌、抗真菌和抗病毒活性的鱼素类化合物对C.刺激物。结果：未经处理的幼蜂和滋养体文库以及经piscidin（Lc-pis）处理的幼蜂和滋养体文库分别产生了57.12、50.44、55.86和47.87万个原始读段。在从头组装后，产生了平均长度为420 bp的966，609个单一基因：其中，618，629个单一基因显示出与一个或多个数据库中的序列相同，其中一些显示出被Lc-pis处理显著操纵。种间分类结果表明，营养体与大黄鱼的同源性在25.8%以上，而营养体与大黄鱼的同源性在3.8%以下。同源单基因表明，寄主组织可以存在于滋养体中，并可能通过囊泡运输到寄生虫体内。我们的分析表明，调控转录参与囊泡贩运。在Lc-pis诱导的转录产物中，大多数基因在处理和未处理的细胞中表达上调，涉及细胞迁移和凋亡相关通路。在处理和未处理的滋养体中，很少发现与处理后的细胞结构和迁移相关的转录物下调。本研究为进一步研究阳离子抗菌肽对纤毛虫的作用机制提供了新的思路。我们全面的转录组分析可以促进确定潜在的药物靶点和疫苗候选人，以控制这种毁灭性的鱼类病原体。
Background:Cryptocaryon irritans is an obligate parasitic ciliate protozoan that can infect various commercially important mariculture fish species and cause high lethality and economic loss. Current methods of controlling this parasite with chemicals or antibiotics are widely considered to be environmentally harmful. Piscidins with broad spectrum antibacterial, antifungal and antiviral activities were found to have potent activity against C. irritans. Little, however, has been understood about the killing mechanisms of piscidins in parasites.Results:In total, 57.12, 50.44, 55.86 and 47.87 million raw reads were generated from untreated theront and trophont, and piscidin (Lc-pis) treated theront and trophont libraries, respectively. After de novo assembly, 966,609 unigenes were generated with an average length of 420 bp: among these, 618,629 unigenes showed identity with sequences in one or more databases, with some showing to be significantly manipulated by Lc-pis treatment. The species classification showed that more than 25.8% unigenes from trophonts were homologous to the large yellow croaker (Larimichthys crocea) and less than 3.8% unigenes from theronts were matched. The homologous unigenes demonstrated that the tissue from host could exist in trophonts and might be transported to parasite via vesicular transports. Our analysis showed that regulatory transcripts were involved in vesicular trafficking. Among transcripts induced by Lc-pis, most genes up-regulated in treated and untreated theronts were involved in cell migration and apoptosis related pathways. Few transcripts were found to be down-regulated in treated and untreated trophonts related to cell structure and migration after treatment.Conclusions:This is the first transcriptome analysis of C. irritans exposed to Lc-pis, which enhanced the genomic resources and provided novel insights into molecular mechanisms of ciliates treated by cationic antimicrobial peptide. Our comprehensive transcriptome analysis can facilitate the identification of potential drug targets and vaccines candidates for controlling this devastating fish pathogen.