MICROWAVE IRRADIATION AS A FORM OF FIXATION FOR LIGHT AND ELECTRON-MICROSCOPY
MICROWAVE IRRADIATION AS A FORM OF FIXATION FOR LIGHT AND ELECTRON-MICROSCOPY
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DOI：
10.1002/path.1711460404
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发表时间：
1985-01-01
影响因子：
7.3
通讯作者：
MILIOS, J
中科院分区：
文献类型：
--
作者：
LEONG, ASY;DAYMON, ME;MILIOS, J
微波辐射被用来固定广泛的手术和尸检标本，以及组织从新鲜杀死的大鼠。尽管不同组织的最佳固定温度似乎各不相同，但从实用的角度来看，将浸入生理盐水中的组织加热至58°C，其固定质量与传统固定的质量相当。10%福尔马林。微波照射施加到组织，其中有高达1小时前浸泡在10%的福尔马林也产生良好的保存细胞形态，微波固定所需的时间不超过150秒。这种形式的快速热固定对特殊染色剂、切片以及对照固定块没有有害影响，并且比常规福尔马林固定产生更少的收缩伪影。更稳定的胞浆抗原的免疫细胞化学染色显示，微波固定与福尔马林固定之间没有显着差异。然而，更不稳定的膜相关抗原的淋巴细胞，没有保存的固定方法。微波固定也被认为是适用于电子显微镜。浸泡在2.5%戊二醛中并在90 s内通过50°C照射固定的组织样本显示出极好的超微结构形态保存。
Microwave irradition was used to fix a wide range of surgical and autopsy specimens as well as tissue from freshly killed rats. Although there appeared to be varying optimum temperatures of fixation for different tissues, from a practical standpoint, the heating to 58°C of tissues submerged in normal saline resulted in fixation of a quality comparable with that produced by conventional fixation to 10 per cent formalin. Microwave irradiation applied to tissues which had up to 1 h prior immersion in 10 per cent formalin also produced excellent preservation of cytomorphology, the time required for microwave fixation being no more than 150 s. This form of rapid heat fixation had no deleterious effects on special stains, sectioned as well as control fixed blocks and produced less shrinkage artefact than conventional formalin fixation. Immunocytochemical staining for more stable cytoplasmic antigens revealed no significant difference between microwave fixation compared with formalin fixation. The more labile membrane associated antigens of lymphocytes, however, were not preserved by either method of fixation. Microwave fixation was also found to be applicable to electron microscopy. Tissue samples immersed in 2.5 per cent glutaraldehyde and fixed in 90 s by irradiation to 50°C showed excellent preservation of ultrastructural morphology.