Molecular characterization and phylogenetic analysis of the eukaryotic translation initiation factor 4A gene in Antheraea pernyi (Lepdoptera: Saturniidae).
Molecular characterization and phylogenetic analysis of the eukaryotic translation initiation factor 4A gene in Antheraea pernyi (Lepdoptera: Saturniidae).
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柞蚕（唇翅目：土星科）真核翻译起始因子 4A 基因的分子特征和系统发育分析
DOI：
10.1093/jisesa/ieu030
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Chen MM;Liu YQ;Li Y;Yao R;Chen M;Xia RX;Li Q;Qin L
真核起始因子4A（eIF-4A）是真核生物蛋白质翻译的重要组成部分。eIF-4A基因（ApeIF-4A）是从柞蚕（Antheraea pernyi）（Guérin-Méneville）（鳞翅目：天蚕科）中分离和鉴定的。获得的cDNA序列长1，435-bp，开放阅读框为1，266 bp，编码421个氨基酸。预测的氨基酸序列与已知的eIF-4As具有一些保守的特征，与智人的eIF-4As的同源性分别为74%和78%。和黑腹果蝇（Drosophila melanogaster（Meigen））。逆转录-聚合酶链反应（RT-PCR）分析表明，ApeIF-4A在4个发育阶段和所有组织中都有转录，表明它在A. pernyi。同源序列比对表明eIF-4As在真核生物进化过程中高度保守。基于eIF-4A的氨基酸和核苷酸序列构建的系统发育树与经典系统学的拓扑结构相似，表明其在真核生物系统发育推断中具有潜在的应用价值。
Eukaryotic initiation factor 4A (eIF-4A) is an essential component for protein translation in eukaryotes. The eIF-4A gene ( ApeIF-4A ) was isolated and characterized from Antheraea pernyi (Guérin-Méneville) (Lepidoptera: Saturniidae). The obtained cDNA sequence was 1,435-bp long with an open reading frame of 1,266 bp encoding 421 amino acids. The predicted amino acid sequence shared several conserved features as found in known eIF-4As and revealed 74 and 78% identities with eIF-4As of Homo sapiens L. and Drosophila melanogaster (Meigen), respectively. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that ApeIF-4A was transcribed at four developmental stages and in all tissues tested, suggesting that it plays an important role in development of A. pernyi . Homologous alignment suggested that eIF-4As are highly conserved throughout evolution of eukaryote organisms. Phylogenetic trees based on the amino acid and nucleotide sequences of eIF-4A demonstrated a similar topology with the classical systematics, suggesting that it has the potential value in phylogenetic inference of eukaryotes.
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DOI：
10.1673/031.010.14140
发表时间：
2010
期刊：
Journal of insect science (Online)
影响因子：
--
作者：
Liu Y;Li Y;Li X;Qin L
通讯作者：
Qin L
影响因子：
4
作者：
Wang, Gen-Hong;Xia, Qing-You;Zhu, Li
通讯作者：
Zhu, Li
影响因子：
14.9
作者：
METZENBERG, S;JOBLET, C;AGABIAN, N
通讯作者：
AGABIAN, N
影响因子：
2.3
作者：
Sun, Shou-Hui;Li, Yu-Ping;Liu, Yan-Qun
通讯作者：
Liu, Yan-Qun
影响因子：
5.3
作者：
PAUSE, A;METHOT, N;SONENBERG, N
通讯作者：
SONENBERG, N